基于气质联用技术的猴头菇多糖抗疲劳代谢组学

Antifatigue Effects of Hericium erinaceus Polysaccharide Using a Metabolomics Technique Based on Gas Chromatography-mass Spectrometry

猴头菇为珍贵的药食两用大型真菌,有抗炎、抗肿瘤、增加免疫力等功效。应用气相色谱-质谱联用(gas chromatography-mass spectrometer,GC-MS)代谢组学方法考察猴头菇多糖对大鼠负重力竭游泳模型的抗疲劳作用及作用机制。将大鼠随机分为6组(空白组、模型组、猴头菇多糖低、中、高3个剂量实验组、阳性对照组),每组6只,连续灌胃21 d;通过测量各组大鼠体重、比较大鼠力竭游泳时间、分析大鼠血清5-HT含量、比较大鼠外周血血象及胸脾指数等实验指标来评估猴头菇多糖对大鼠疲劳的缓解程度;采用GC-MS技术分析各组大鼠血清中代谢物,并进行多元统计分析获取差异性代谢物信息,构建代谢通路并阐述抗疲劳作用机制。结果表明:猴头菇多糖高剂量组的大鼠负重力竭游泳时间显著长于模型组;猴头菇多糖低、中、高剂量组大鼠代谢轮廓趋近于空白组,表现出一定抗疲劳作用。获得了24个差异性代谢物与负重力竭游泳模型有关,涉及的3条主要代谢通路为:亚油酸代谢、甘油代谢及苯丙氨酸、酪氨酸、色氨酸合成。推测猴头菇多糖可能通过调节脂质代谢以及氨基酸代谢对负重力竭游泳模型大鼠发挥抗疲劳作用。

Hericium erinaceus is a valuable medicinal and edible macrofungus,which has anti-inflammatory,anti-tumor,and immunity-increasing effects.Gas chromatography-mass spectrometer(GC-MS)metabolomics was used to investigate the anti-fatigue effect and mechanism interpretation of Hericium erinaceus polysaccharide on rat negative gravity exhaustion swimming model.The rats were randomly divided into 6 groups(blank group,model group,3 experimental groups with low,medium and high doses of Hericium erinaceus polysaccharides,and positive control group),with 6 rats in each group.After continuous gastric gavage for 21 days,GC-MS technology was used to analyze metabolites in the serum of rats in each group,and the differential metabolite information was obtained by multivariate statistical analysis,and the anti-fatigue effect of metabolic pathway was constructed to explain.The results show that the swimming time of rats in the high-dose group of Hericium erinaceus polysaccharide is significantly longer than that in the model group.The metabolic profile of rats in the dosing group is close to that of the blank group,showing certain anti-fatigue effects.The differential metabolites in serum 24 are identified as related to the negative gravity exhaustion swimming model,involving three major metabolic pathways:linoleic acid metabolism,glycerol metabolism,phenylalanine,tyrosine and tryptophan biosynthesis.It is hypothesized that Hericium erinaceus polysaccharides may exert anti-fatigue effects on negative gravity-exhausted swimming model rats by regulating lipid metabolism.