昆虫溶茧酶基因的初步鉴定与演化分析

Identification and Preliminary Evolution Analysis of Insect Cocoonase

溶茧酶(cocoonase)是研究昆虫演化及其生理适应性的重要酶类。通过The MEME Suite分析得到4条昆虫溶茧酶特征基序,利用HMMER和BLAST程序分析了105种昆虫的溶茧酶基因分布情况,但是仅在36种鳞翅目昆虫中鉴定到了溶茧酶的编码基因,特别是在蛱蝶科(如袖蝶等)昆虫里有明显的扩增条带。进一步通过Gromacs软件初步模拟溶茧酶及其相似的胰蛋白酶在家蚕唾液中的活性与稳定性,结果发现溶茧酶的最终能量更低,表明其更适应家蚕吐出液环境。以上鉴定与分析结果为进一步研究昆虫溶茧酶的演化模式和生理功能积累了一定的基础信息。

The relationship between the evolution of cocoonases in insects and their physiological adaptability is a research hotspot in biological evolution.However,the sequence characteristics and identification of cocoonase are still unclear.In this study,the MEME Suite analysis was used to obtain 4 signature motifs of cocoonase.The distribution of cocoonase in 105 insects was analyzed by HMMER and BLAST programs.The results showed that this gene was identified only in 36 species of Lepidoptera insects,and even significant gene duplication was found especially in the Nymphalidae(such as Pteropodidae).The activity and stability of cocoonase and trypsin in silkworm saliva were initially simulated by Gromacs.As a result,the final energy of cocoonase was lower,indicating that it was more suitable for the environment of silkworm spit.These results laid the foundation for further analysis of the evolutionary and function of cocoonase.