高压电烧伤大鼠肝脏氧化应激损伤及灯盏花素的干预作用

Hepatic oxidative stress injury in high-voltage electrical burns rats and the intervention effect of brevisanthin

目的探讨氧化应激在大鼠高压电烧伤肝损伤过程中的作用及灯盏花素的干预效果。方法本研究分为电伤实验和治疗实验两部分。采用随机数字表法将170只雄性SD大鼠分配至电伤实验(120只)和治疗实验(50只)。将电伤实验分为电伤组(60只)和假伤组(60只),观测时相为电伤后0、8、24、48、72 h及1周,每个观测时相10只。将治疗实验分为高、中、低剂量组,电伤组和盐水组,每组10只。采用实验高压电击系统制作电压3 kV、电击3 s的大鼠高压电烧伤模型。电伤实验大鼠按时相采集血清及肝脏组织。治疗实验大鼠高、中、低剂量组分别腹腔注射6 mg/kg、4 mg/kg和2 mg/kg灯盏花素,盐水组腹腔注射生理盐水1mL/kg,电伤组作空白对照,治疗72 h后处死并采集血清及肝脏组织。光镜下观察肝组织形态学变化,检测血清中总胆红素(TBIL)含量,谷丙转氨酶(ALT)、谷草转氨酶(AST)活性,检测肝脏匀浆上清中还原型谷胱甘肽(GSH)、超氧阴离子(O2-·)、总巯基(-SH)含量,黄嘌呤氧化酶(XOD)活性及羟自由基(·OH)清除率。结果高压电烧伤大鼠肝组织进行性微循环障碍及炎性细胞浸润,使用灯盏花素治疗后肝损伤减轻。电伤实验中,电伤组TBIL、O2-·含量和·OH清除率与假伤组比较,差异无统计学意义(F=0.184、0.485、0.119,P=0.678、0.504、0.739);电伤组ALT、AST和XOD活性高于假伤组,差异有统计学意义(F=215.500、92.010、65.400,P<0.001);电伤组-SH和GSH含量低于假伤组,差异有统计学意义(F=27.890、113.500,P<0.001)。治疗实验组间相互比较,TBIL、O2-·含量和·OH清除率差异无统计学意义(F=1.441、1.890、0.249,P=0.236、0.129、0.909);与电伤组和盐水组比较,中剂量组ALT、AST、XOD活性和-SH含量降低,GSH含量升高,差异有统计学意义(P<0.05)。结论高压电烧伤导致大鼠肝脏组织和细胞损伤,可能与氧化应激相关。灯盏花素对高压电烧伤大鼠肝脏损伤和氧化应激有改善作用。

Objective To investigate the role of oxidative stress in liver injury caused by high-voltage electrical burns in rats and the intervention effect of brevisanthin.Methods The study was divided into electrotrauma experiment and treatment experiment.A total of 170 male SD rats were assigned to the electrotrauma experiment(n=120)and the treatment experiment(n=50)using the random number table method.The electrotrauma experiment was divided into electrical injury group(n=60)and sham electrical injury group(n=60),and the observation phases were 0,8,24,48,72 h and 1 week after electrical injury,and 10 animals were observed in each observation phase.The treatment experiment was divided into high-dose,medium-dose and low-dose treatment groups,electrical injury group and saline group,with 10 animals in each group.The experimental high-voltage electrical shock system was used to make high-voltage electrical burn model of rats with a voltage of 3 kV and an electric shock of 3 seconds.Electrotrauma experimental rats were collected serum and liver tissue on time.The high-dose,medium-dose,and low-dose groups were intraperitoneally injected with 6 mg/kg,4 mg/kg,and 2 mg/kg brevisanthin,respectively,the saline group was intraperitoneally injected with 1 mL/kg of normal saline,and the electrical injury group was used as blank control,and the serum and liver tissue were sacrificed and collected after 72 hours of treatment.The morphological changes of liver tissue were observed under light microscopy,the content of total bilirubin(TBIL)and glutamic-pyruvic transaminase(ALT),glutamic-oxaloacetic transaminase(AST)activity in serum were measured,and the contents of glutathione(GSH),superoxide radical(O2-·)and total sulfhydryl(-SH),xanthine oxidase(XOD)activity and hydroxyl radical(·OH)scavenging rate in the supernatant of liver homogenate were detected.Results Progressive microcirculatory disorders and inflammatory cell infiltration occurred in liver tissue of high-voltage electrical burns rats,liver damage was reduced after treatment with brevisanthin.In the electrotrauma experiment,there was no significant difference in the content of TBIL,O2-·and·OH scavenging rate between the electrical injury group and the sham electrical group(F=0.184,0.485,0.119;P=0.678,0.504,0.739).The ALT,AST,XOD activity in the electrical group were higher than those in the sham electrical group,the difference was significant(F=215.500,92.010,65.400,P<0.001).The content of GSH and-SH in the electrical injury group were lower than those in the sham electrical group,and the difference was significant(F=27.890,113.500,P<0.001).There was no significant difference in the content of TBIL,O2-·and·OH scavenging rate among treatment experimental groups(F=1.441,1.890,0.249;P=0.236,0.129,0.909).The ALT,AST,XOD activity and the content of-SH in the middle-dose group decreased and the content of GSH increased,and the difference was significant(P<0.05).Conclusion High-voltage electrical burns cause liver tissue and cell damage in rats,which may be associated with oxidative stress.Brevisanthin has an ameliorating effect on liver injury and oxidative stress in rats with high-voltage electrical burns.