云南分心木对小鼠肾缺血-再灌注损伤的防护作用研究

Protective effect of renal ischemia-reperfusion injury of Yunnan Diaphragma Juglandis Fructus in mice

目的探讨云南分心木中部分金属元素与小鼠双侧夹闭肾缺血-再灌注损伤(renal ischemia reperfusion injury,RIRI)防护作用的相关性,为明确分心木传统药用基础提供依据。方法利用强酸性阳离子交换树脂制备得去离子分心木水提物;运用电感耦合等离子体发射光谱法(ICP-AES)测定分心木醇提物、水提物和去离子水提物中7种金属元素的含量;采用双侧夹闭法构建肾缺血-再灌注损伤小鼠模型,对分心木提取物中部分金属元素与RIRI小鼠的生化指标进行相关性分析。结果云南分心木提取物各剂量组均能不同程度的降低肾缺血-再灌注小鼠血清肌酐(CRE)和尿素氮(BUN)水平,下调肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)的水平,提取物中Mg、Zn元素含量与RIRI小鼠模型血清中CRE含量呈(负)显著相关;Mn元素含量与BUN呈(负)极显著相关,与IL-1β的水平呈负相关;Fe元素含量与TNF-α的水平呈负相关,显示分心木中部分金属元素具有防护RIRI作用。结论云南分心木中部分金属元素与其发挥防护小鼠双侧夹闭肾缺血-再灌注损伤作用具有相关性,在一定范围内具有防护RIRI的作用。

Objective To explore the correlation between some metallic elements of Diaphragma Juglandis Fructus(DJF)from Yunnan and the protective effect of bilateral clamped renal ischemia-reperfusion injury(RIRI)in mice,and to provide the basis for clarifying the traditional medicinal material basis of distractor.Methods The deionized aqueous extract of DJF from Yunnan was prepared by using strong acid cation exchange resin.The contents of seven metallic elements in the deionized aqueous extract of DJF,as well as in aqueous extract of DJF and ethanol extract of DJF,were measured by inductively coupled plasma atomic emission spectrometry(ICP-AES).The mouse model of RIRI was established by bilateral clamping method,and the correlation between some metallic elements in DJF extracts and biochemical indexes of RIRI mice was analyzed.Results Every group of DJF extract could reduce the levels of serum creatinine(CRE)and urea nitrogen(BUN)in mice with RIRI,reducing IL-1βand TNF-αlevel.The contents of Mg and Zn in the extracts were(negatively)significantly correlated with the content of CRE of RIRI mouse model;the content of Mn was(negatively)significantly correlated with BUN and IL-1β.The expression level was negatively correlated with the content of Fe and TNF-α.The experimental results showed that some metallic elements in DJF had the protective effect on RIRI.Conclusion Some metallic elements of DJF are related to their role in preventing bilateral clamped RIRI in mice,and can protect RIRI in a certain range.