去氧地胆草素对人大细胞肺癌H460细胞株的抑制作用研究

Study on the anti-tumor effect of deoxyelephantopin on large cell lung cancer H460 cells

为进一步研究去氧地胆草素(Deoxyelephantopin,DET)对人大细胞肺癌H460细胞株的抑制作用,本研究通过网络药理学分析,确定去氧地胆草素影响人大细胞肺癌H460细胞株的潜在信号通路。再通过细胞增殖和凋亡实验,研究不同浓度去氧地胆草素对人大细胞肺癌H460细胞株增殖和凋亡的影响。网络药理学分析表明,去氧地胆草素可能通过影响凋亡相关蛋白诱发细胞凋亡从而抑制细胞增殖。MTT和细胞凋亡实验结果显示,去氧地胆草素能有效抑制H460细胞的增殖,能促进H460细胞的凋亡,且均呈剂量依赖效应。

In order to further investigate the inhibitory effect of Deoxyelephantopin(DET)on H460 cell line of human cell lung cancer,this study employed network pharmacological analysis to identify potential signaling pathways of DET affecting hu-man cell lung cancer H460 cell line.Cell proliferation and apoptosis experiments were then conducted to examine the effects of different concentrations DET on proliferation and apoptosis of H460 cell line of human cell lung cancer.Network pharmacologi-cal analysis showed that DET may inhibit cell proliferation by affecting apoptosis-related proteins to induce apoptosis.The re-sults of MTT and apoptosis experiments showed that DET could effectively inhibit the proliferation of H460 cells and promote the apoptosis of H460 cells in a dose-dependent manner.