基于一测多评、化学计量学和EW-TOPSIS法的三叶青药材质量差异评价研究

Quality difference evaluation of Radix tetrastigmae based on quantitative analysis of multi-components by single-marker,chemometrics and entropy weight-technique for order preference by similarity to ideal solution method

目的采用一测多评(QAMS)、化学计量学和熵权-优劣解距离(EWTOPSIS)法对不同产地三叶青进行质量差异评价,为其道地研究提供依据。方法收集重庆、广西、湖南、广东、浙江、江苏等6省18批次三叶青样品,采用HPLC-QAMS法同时检测三叶青中绿原酸、咖啡酸、儿茶素、原花青素B1、荭草苷、芦丁、虎杖苷、异槲皮素、山奈酚-3-O-芸香糖苷、紫云英苷、白藜芦醇和山柰酚的含量,建立三叶青多组分定量控制模式;采用主成分分析和正交偏最小二乘法-判别分析等化学模式识别筛选影响三叶青药材质量的主要潜在标志物;利用EW-TOPSIS法建立三叶青质量优劣评价模型,对其质量差异性进行综合评价。结果12个成分在各自范围内线性关系良好,平均加样回收率为96.89%~100.13%,RSD<2.0%(n=9);HPLC-QAMS法计算值与外标法实测值之间无明显差异;前2个主成分累计方差贡献率为90.15%;原花青素B1、儿茶素、芦丁、荭草苷和绿原酸可能是影响三叶青产品质量主要潜在标志物;EW-TOPSIS法分析结果显示18批三叶青的相对关联度为0.1549~0.7320。结论所建立的方法操作便捷、结果准确,可作为三叶青多组分定量控制方法;化学计量学及EW-TOPSIS法客观全面,可用于三叶青的质量差异评价。

Objective To evaluate the quality difference of Radix tetrastigmae from different producing areas by quantitative analysis of multi-components by single-marker(QAMS),chemometrics and entropy weight-technique for order preference by similarity to ideal solution(EW-TOPSIS)method,and to provide basis for studying the geoherbalism of Radix tetrastigmae.Methods 18 batches of Radix tetrastigmae samples were collected from 6 provinces including Chongqing,Guangxi,Hunan,Guangdong,Zhejiang and Jiangsu.The contents of chlorogenic acid,caffeic acid,catechin,procyanidin B1,orientin,rutoside,polydatin,isoquercitrin,kaempferol-3-O-rutinoside,astragalin,resveratrol and kaempfero in Radix tetrastigmae were simultaneously determined by HPLC-QAMS,and a multi-component quantitative control mode for Radix tetrastigmae was established.The main potential markers affecting the quality of Radix tetrastigmae were screened by chemical pattern recognition such as principal component analysis and orthogonal partial least squares-discriminant analysis.The EW-TOPSIS was used to establish the quality evaluation model of Radix tetrastigmae,and the quality difference was comprehensively evaluated.Results The 12 components showed good linear relationships in their own ranges.The average recovery were 96.89%-100.13%,with the RSDs﹤2.0%(n=9).There was no significant difference between the calculated values of HPLC-QAMS and the measured values of external standard method.The cumulative variance contribution rate of the first two principal components was 90.15%.The proanthocyanidin B1,catechin,rutoside,orientin and chlorogenic acid may be the main potential markers affecting the quality of Radix tetrastigmae.The results of EW-TOPSIS analysis showed that the relative correlation of 18 batches of Radix tetrastigmae were 0.1549-0.7320.Conclusion The established method is convenient in operation and accurate in results,and can be used as a quantitative control method for multi-components of Radix tetrastigmae.The chemometrics and EW-TOPSIS method are objective and comprehensive,and can be used for the overall quality difference evaluation of Radix tetrastigmae.