食管癌患者SOX7、KLK13、FHIT的表达及对术后复发转移的预测价值

Expression of SOX 7,KLK13 and FHIT in patients with esophageal cancer and its value in prediction of postoperative recurrence and metastasis

目的:探讨SRY盒相关基因7(SOX7)、激肽释放酶相关肽酶13(KLK13)、脆性组氨酸三联体基因(FHIT)在食管癌患者癌组织中的表达,并分析其与术后复发转移的关联性。方法:选取2022年1月—2023年1月某院行胸腔镜食管癌切除术治疗的86例食管癌患者为研究对象,术后随访1年(失访5例),根据随访结局将患者分为复发转移组(31例)和未复发转移组(50例)。比较不同组织(癌组织vs癌旁组织)、不同结局及不同病理学参数的食管癌患者癌组织中SOX7、KLK13、FHIT mRNA表达水平。绘制ROC曲线,分析癌组织各指标联合检测对食管癌患者术后复发转移的预测价值。结果:食管癌患者癌组织SOX7、KLK13、FHIT mRNA表达低于癌旁组织,差异有统计学意义(P<0.05);复发转移组患者癌组织SOX7、KLK13、FHIT mRNA低于未复发转移组,差异有统计学意义(P<0.05)。分化程度为中高分化的食管癌患者癌组织中SOX7、KLK13、FHIT mRNA表达水平高于分化程度为低分化的食管癌患者,肿瘤分期为Ⅲ期、伴有淋巴结转移的食管癌患者癌组织中SOX7、KLK13、FHIT mRNA表达水平低于肿瘤分期为Ⅰ~Ⅱ期、不伴有淋巴结转移的食管癌患者,差异有统计学意义(P<0.05)。ROC曲线分析结果显示,癌组织SOX7、KLK13、FHIT mRNA预测食管癌患者术后复发转移的AUC分别为0.793、0.829、0.776,三者联合预测食管癌患者术后复发转移的AUC为0.909。结论:食管癌患者癌组织中SOX7、KLK13、FHIT mRNA表达降低,其表达水平受肿瘤分期、分化程度、淋巴结转移及术后复发转移影响,联合预测食管癌术后复发转移结局的效能较高,可作为拟定诊疗计划的参考。

Objective:To explore the expression of sex determining region Y(SRY)box 7(SOX7),recombinant kallikrein 13(KLK13),and fragile histidine triad(FHIT)gene in tissues of patients with esophageal cancer,and to analyze its correlation with postoperative recurrence and metastasis.Methods:86 patients with esophageal cancer undergoing esophagectomy in a hospital from January 2022 to January 2023 were chosen as objectives,and were followed up for one year(5 cases missing in the follow-up).According to the result of the follow-up,the patients were divided into a recurrence/metastasis group(n=31)and a non-recurrence/metastasis group(n=50).mRNA expressions of SOX7,KLK13 and FHIT in different tissues(cancer tissues vs paracancerous tissues),results and pathological parameters in cancer tissues of the patients with esophageal cancer were compared.ROC was drawn to analyze the predictive value of joint detection of different indicators on postoperative recurrence and metastasis of the patients with esophageal cancer.Results:The mRNA expressions of SOX7,KLK13 and FHIT in cancer tissues of the patients were remarkably lower than those in paracancerous tissues(P<0.05).The mRNA expressions of SOX7,KLK13 and FHIT in cancer tissues of the recurrence/metastasis group were remarkably lower than those in the non-recurrence/metastasis group(P<0.05).The mRNA expressions of SOX7,KLK13 and FHIT in cancer tissues of the moderately/well differentiated patients with esophageal cancer were higher than those in the poorly differentiated patients.The mRNA expressions of SOX7,KLK13 and FHIT in cancer tissues of stage III of the esophageal cancer patients with lymphatic metastasis were lower than those of stage I or II patients without lymphatic metastasis(P<0.05).ROC analysis thowed that the mRNA expressions of SOX7,KLK13 and FHIT in cancer tissues in AUC to detect the postoperative recurrence and metastasis of the patients were respectively 0.793,0.829 and 0.776;the AUC in the joint detection of the three in prediction of the postoperative recurrence and metastasis of the patients was 0.909.Conclusion:The mRNA expressions of SOX7,KLK13 and FHIT in cancer tissues of patients with esophageal cancer decrease.Its expression level is influenced by tumor staging,degree of differentiation,lymphatic metastasis and postoperative recurrence and metastasis.Joint detection of postoperative recurrence and metastasis of esophageal cancer is of high effect,which can be taken as reference for the further diagnosis and treatment.