海参蒸煮液对慢性便秘小鼠的通便作用

Effect of Sea Cucumber Cooking Liquid on Chronic Constipation Mice

目的:探究海参蒸煮液(SCCL)对小鼠慢性便秘的改善作用。方法:以盐酸洛哌丁胺构建小鼠慢性便秘模型,检测SCCL干预对慢性便秘小鼠排便、小肠推进率以及肠动力相关神经递质和受体基因表达的影响。结果:相较于模型对照组,SCCL高剂量组(500 mg/kg BW)小肠墨汁推进率显著增加(P<0.05),首次排便时间极显著缩短(P<0.01),SCCL低、高剂量组(250,500 mg/kg BW)6 h内排黑便粒数和质量均显著增加(P<0.05);小鼠结肠组织中杯状细胞数量、黏蛋白含量均显著增加(P<0.05);M_(2)AChR、M_(3)AChR、5-HT_(3)R、5-HT_(4)R以及Cajal间质细胞(ICC)干细胞因子C-KitmRNA表达显著升高(P<0.05)。结论:SCCL通过调节与肠道蠕动的相关神经递质ACh和5-HT及其下游信号通路,增加ICC细胞数量,提高肠动力,促进肠道蠕动,从而达到改善便秘的效果。

Objective:To investigate the effect of sea cucumber cooking liquid(SCCL)dry powder on chronic constipation in mice.Methods:Loperamide hydrochloride was administered intragastrically to establish the chronic constipation model.To evaluate the positive effect of SCCL in intestinal motility of constipation mice,the defecation situation,intestinal ink transit rate and mRNA expression levels of intestinal motility related neurotransmitters and receptors were detected.Result:Compared with the model control group,the intestine ink transit rate of mice in the high-dose SCCL group was significantly increased(P<0.05),the time of first black faeces was significantly shortened(P<0.01),and the number and weight of faeces in 6 hours in the low-dose and high-dose SCCL groups were significantly increased(P<0.05);The number of goblet cells and the content of mucin in the colon of mice were improved significantly(P<0.05);The mRNA expression levels of M_(2)AChR,M_(3)AChR,5-HT_(3)R,5-HT_(4)R and C-Kit in colon tissue were significantly increased(P<0.05).Conclusion:SCCL alleviated constipation by regulating intestinal peristalsis related neurotransmitters such as ACh,5-HT and its downstream signaling pathways,eventually increased the number of ICC cells,improved intestinal motility and promoted intestinal peristalsis.