多酸基金属-有机复合物的合成和对H_(2)O_(2)的检测

Synthesis and H_(2)O_(2)Detection of POMs Based Metal-organic Framework Compounds

设计合成多酸基金属-有机晶态复合物,研究其对于过氧化氢小分子的催化活性,为开发新型生物小分子传感器提供新途径。利用水热法合成和X-射线单晶衍射表征多酸基金属有机晶态复合物;利用紫外-可见分光光度研究其对过氧化氢小分子的催化活性。成功制备并解析出多酸基金属有机晶态复合物,其分子式为H_(4)[Cu_(2)(bim)_(4)][SiW_(12)O_(40)]_(2)·8H_(2)O,拟酶活性研究表明复合物具有过氧化酶活性,其最优催化条件为:pH=3,40℃,反应时间为4min,对过氧化氢的检测区间为1~100μmol×L^(-1),检测限为0.18μmolL^(-1)。多酸基金属-有机晶态复合物尽管对于生物小分子传感表现出优异的检测性能,但是其种类和结构对其催化活性是有一定的影响,其构效关系还需要进一步深入研究。

Objective:To design and synthesize POMs based metal-organic crystalline compounds,and study their catalytic activity for H_(2)O_(2),and provide a new approach for the development of new biological small molecule sensors.Method:Hydrothermal synthesis and X-ray single crystal diffraction were used to characterize POMs based metal-organic crystalline complexes.And UV-visible spectrophotometry was employed to study the catalytic activity of POMs based metal-organic crystalline compounds to H_(2)O_(2).Result:POMs based metal-organic crystalline compound was successfully prepared,and X-ray single diffraction analysis shows that the molecular formula is H 4[Cu_(2)(bim)4][SiW_(12)O_(40)]_(2)·8H_(2)O.Studies on enzymatic activity shows that the compound possesses peroxidase-like activity,and the optimal catalytic conditions are pH=3,40℃,reaction time of 4 minutes,and the detection range for H_(2)O_(2)is 1~100μmolL^(-1)with the detection limit of 0.18μmolL^(-1).Conclusion:POMs based metal-organic crystalline compounds exhibit excellent detection performance for biological small molecule sensing,but their types and structures have a certain impact on their catalytic activity,and their structure-activity relationship still needs further in-depth research.