摘要
目的通过比较寻常痤疮皮损和健康人毛囊内表皮葡萄球菌(SE)的基因分型,探索SE在痤疮发生中的作用。方法本研究为横断面研究,于2022年8月至2023年8月纳入上海交通大学医学院附属仁济医院皮肤科就诊的中度寻常痤疮(简称痤疮)患者和健康志愿者。分离培养痤疮患者脓疱内容物和健康志愿者毛囊中SE菌株,并通过聚合酶链反应进行管家基因扩增、测序和多位点序列分型,获得菌株的序列类型(ST),比较不同来源菌株的基因型、亲缘关系。结果痤疮组患者28例,年龄(22.6±2.6)岁,男女比例为10∶18;健康对照组志愿者19例,年龄(22.4±0.96)岁,男女比例为7∶12。两组间年龄和性别比例差异均无统计学意义(均P>0.05)。痤疮组和健康对照组皮肤标本SE检出率分别为60.71%(17/28)和73.68%(14/19),差异无统计学意义(P=0.53)。来自健康对照组的144株SE可以被分为10个ST型,其中检出率最高的5个ST型依次是ST35(8例)、ST73(4例)、ST193(2例)、ST59(2例)、ST540(2例);分离自痤疮组的190株SE可被分为16个ST型,其中检出率最高的5个ST型依次是ST59(6例)、ST73(6例)、ST802(3例)、ST130(3例)、ST35(2例)。痤疮组ST35的检出率显著低于健康对照组(P=0.018),其余ST型的检出率差异无统计学意义(P>0.05)。分析SE菌株之间的亲缘关系发现分别位于3个进化树分支上,健康对照组的SE菌株大部分属于分支A,痤疮组SE菌株中分支A菌株的比例[M(Q):25%(85%)]显著低于健康对照组[100%(33.33%),U=66,P=0.025],分支B的比例[14.29%(89.17%)]显著高于健康对照组[0(0),U=62,P=0.010],分支D的比例[0(57.14%)]与健康对照组[0(4.17%)]差异无统计学意义(P=0.420)。结论痤疮患者皮损内SE基因分型与健康人群之间存在差异,可能与痤疮发生或者疾病进程有关。
Objective To compare the phylotypes of Staphylococcus epidermidis(SE)in skin lesions of acne vulgaris patients versus hair follicles of healthy people,and to analyze their roles in the pathogenesis of acne vulgaris.Methods A cross-sectional study was conducted from August 2022 to August 2023.Patients with moderate acne vulgaris,as well as healthy volunteers,were enrolled from the Department of Dermatology,Renji Hospital,School of Medicine,Shanghai Jiaotong University.SE strains were isolated from the pustules of acne vulgaris patients and hair follicles of healthy volunteers.Housekeeping genes were amplified by PCR.Sequencing and multilocus sequence typing were performed to compare the phylotypes and genetic relationships of strains from different sources.Results The acne group consisted of 28 patients(10 males and 18 females)with the age being 22.6±2.6 years,while the healthy group consisted of 19 volunteers(7 males and 12 females)with the age being 22.4±0.96 years.There were no significant differences in age or gender ratio between the two groups(both P>0.05).The positive rates of SE in the samples of the acne group and the healthy group were 60.71%(17/28)and 73.68%(14/19),respectively,with no significant difference between the two groups(P=0.53).The 144 SE strains from the healthy group could be divided into 10 sequence types(STs),and the most common ST was ST35(8 cases),followed by ST73(4 cases),ST193(2 cases),ST59(2 cases)and ST540(2 cases);190 SE strains from the acne group could be divided into 16 STs,and the most common STs were ST59(6 cases)and ST73(6 cases),followed by ST802(3 cases),ST130(3 cases)and ST35(2 cases).The positive rate of ST35 was significantly lower in the acne group than in the healthy group(P=0.018),while there were no significant differences in the positive rates of other STs between the two groups(all P>0.05).The evolutionary tree analysis showed that the SE strains were mainly distributed in 3 branches.Most of the SE strains from the healthy group belonged to clade A.The proportion of SE strains in clade A(M[Q])was significantly lower in the acne group(25%[85%])than in the healthy group(100%[33.33%],P=0.025),while the proportion of SE strains in clade B was significantly higher in the acne group(14.29%[89.17%])than in the healthy group(0[0],U=62,P=0.010),and there was no significant difference in the proportion of SE strains in clade D between the acne group(0[57.14%])and healthy group(0[4.17%],P=0.420).Conclusion The phylotypes of SE strains differed between acne vulgaris patients and healthy controls,possibly associated with the occurrence and development of acne vulgaris.
作者
李嘉祺
梁梦晨
吴心怡
张秋婧
李思彤
莫小辉
鞠强
Li Jiaqi;Liang Mengchen;Wu Xinyi;Zhang Qiujing;Li Sitong;Mo Xiaohui;Ju Qiang(Department of Dermatology,Renji Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200127,China)
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2024年第4期295-301,共7页
Chinese Journal of Dermatology
基金
上海交通大学医学院附属仁济医院课题(IIT-2022-0011)
国家自然科学基金(81874247)。
关键词
寻常痤疮
表皮葡萄球菌
毛囊
微生物群落
基因分型技术
多位点序列分型
Acne vulgaris
Staphylococcus epidermidis
Hair follicle
Microbial consortia
Genotyping techniques
Multilocus sequence typing