载有左氧氟沙星的普鲁兰多糖薄膜用于伤口敷料

Pullulan Film Loaded with Levofloxacin for Wound Dressings

以普鲁兰多糖(Pullulan)为原料、三偏磷酸钠(STMP)为交联剂、甘油(Glycerol)为增塑剂,在碱性条件下,制备得到载有抗菌药物左氧氟沙星(Levofloxacin)的普鲁兰多糖复合薄膜。对薄膜的化学结构、透明度、耐水性能、吸水性能、水蒸气透过性能、力学性能、抗菌性以及生物相容性进行了研究。结果表明,当STMP的添加量(与普鲁兰多糖的质量比,余类推)为10%、甘油添加量为30%、NaOH添加量为0.4%、左氧氟沙星添加量为1%时,得到的薄膜具有较高的耐水性、吸水性和透明度,以及良好的力学性能等,在水中浸泡24 h后的质量损失为38.74%,同时对金黄色葡萄球菌和大肠杆菌的抗菌率达到近100%,生物相容性较好。

Film dressings adhere well to the skin,even in the joints of the human body,and can serve as a wound barrier to promote wound healing.To improve water resistance of the film dressings,sodium trimetaphosphate(STMP) was selected to crosslink pullulan.Using glycerol as plasticizer and levofloxacin as antibacterial drug under alkaline condition,the pullulan composite films were prepared.Chemical structure,transparency,water resistance,water absorption,water vapor permeability,mechanical properties,antibacterial properties,and biocompatibility of the pullulan composite films were studied.Results showed that the composite films had high transparency and good water resistance,which not only facilitated the observation of wound healing,but also maintained its integrity during use,avoiding damage caused by the dissolution of exudate and delaying wound healing,when the addition amount(relative to the mass of pullulan,the same below) of STMP was 10%,glycerol was 30%,NaOH was 0.4%,and levofloxacin was 1%.At the same time,the composite films had appropriate water absorption,moisture permeability,and mechanical properties,which could absorb wound exudate,maintain appropriate humidity conditions for the wound healing to facilitate wound healing,and better fit the skin.Moreover,the composite films had 100% antibacterial activity against both Staphylococcus aureus(S.aureus) and Escherichia coli(E.coli),and had good biocompatibility.The resultant pullulan composite films had potential application of wound healing.