林下仿野生栽培和设施栽培金线莲生物量及药用成分含量变化研究

Variation of Biomass and Medicinal Components in Anoectochilus roxburghii with Different Cultivation Methods

以金线莲Anoectochilus roxburghii组培苗为对照,研究仿野生栽培和温室大棚设施栽培3年内金线莲中可溶性多糖和黄酮药用成分含量的变化以及鲜质量与干质量的变化。结果表明:栽培1年后,林下仿野生栽培金线莲可溶性多糖和黄酮含量均高于大棚设施栽培金线莲的含量,且随着栽培年份的增加两种成分的含量均呈下降趋势。栽培3年,设施栽培金线莲植株的鲜质量积累高于林下仿野生栽培金线莲植株;林下仿野生栽培和设施栽培金线莲的干质量含量(干质量/鲜质量)均随时间增加呈现逐渐上升趋势,但林下仿野生栽培金线莲的干质量含量高于设施栽培。综合以上研究结果表明,以金线莲药用成分及天然产物提取为主要目标,推荐林下仿野生栽培1年生金线莲;以鲜生物量为目标,推荐设施栽培金线莲;以干生物量为目标,则推荐林下仿野生栽培方式。

Tissue cultured Anoectochilus roxburghii seedlings were transplanted in nursery of Wenzhou,and under Phyllostachy edulis and under mixed forest of Ph.edulis and Cunninghamia lanceolata in Pingyan,Zhejiang province.In November of the current and the 2nd and 3rd year,determinations were implemented on content of polysaccharide and flavonoids,fresh and dry biomass of seedlings.The results showed that the contents of soluble polysaccharide and flavonoids in understory cultivated A.roxburghii were higher than that in the nursery within the first year,and the contents showed a decreasing trend with the increase of years.Fresh weight increased in the nursery was higher than that understory.Dry weight of understorey cultivated seedlings was higher than that in the nursery.