黑桑枝化学成分研究

Chemical Constituents from Twigs of Morus nigra

目的:研究黑桑枝的化学成分。方法:采用HP20大孔树脂柱、正相硅胶柱、Sephadex LH-20葡聚糖凝胶柱以及半制备型液相色谱等技术对黑桑枝醇提物的乙酸乙酯部位进行分离纯化,运用现代波谱学方法(UV、IR、MS、NMR)鉴定化合物的结构。结果:从黑桑枝中共分离得到9个化合物,分别鉴定为:2′,4′,2″-三羟基查尔酮-4″-O-β-D-葡萄糖苷(1)、槲皮素(2)、2,4-二羟基苯甲醛(3)、二氢槲皮素(4)、3-O-甲基槲皮素(5)、3-O-甲基槲皮素-7-O-β-D-葡萄糖苷(6)、桑黄素(7)、槲皮素-3′-O-β-D-葡萄糖苷(8)、槲皮素-4′-O-β-D-葡萄糖苷(9)。结论:其中,化合物1为一新的查尔酮葡萄糖苷类化合物,化合物1、5、6、9为首次从该植物中分离得到。

Objective:To study the chemical constituents from the twigs of Morus nigra.Methods:The ethyl acetate fraction of Mo-rus nigra twigs was isolated and purified by HP20 macroporous resin column choromatography,normal-phase silica gel,Sephadex LH-20 and semi-preparative liquid chromatography,and the structures were identified by modern spectral methods(UV,IR,MS,NMR).Re-sults:Nine compounds were isolated from the twigs of Morus nigra and identified as 2′,4′,2″-trihydroxychalcone-4″-O-β-D-glucoside(1),quercetin(2),2,4-dihydroxybenzaldehyde(3),taxifolin(4),3-O-methylquercetin(5),3-O-methylquercetin-7-O-β-D-glucoside(6),morin(7),quercetin-3′-O-β-D-glucoside(8),quercetin-4′-O-β-D-glucoside(9),respectively.Conclusion:Among them,compound 1 is a new chalcone glucoside,compounds 1,5,6 and 9 are isolated from Morus nigra for the first time.