基于Meta分析菌根菌对植物叶片生理的影响

Meta-analysis of the effects of mycorrhizal fungi on plant leaf physiology

叶片作为植物的重要器官,其养分、光合色素、可溶性糖、可溶性蛋白等含量,以及各种酶活性都是表征其生理的重要参数;而光合作用、蒸腾作用则是叶片重要的生理活动指标。菌根菌是一类与绝大多数植物形成共生的土壤微生物,能够影响叶片生理。本研究选择分布最广泛的丛枝菌根菌(arbuscular mycorrhizal fungi, AMF),针对以往大多数研究只关注单一植物接种AMF探究其生理功能的问题,采用Meta分析(Meta-analysis)的定量研究方法,对152篇相关文献整理建库,评估了AMF对植物叶片生理的影响,探究了不同分类水平上AMF的效应。结果表明:AMF显著提高了叶片氮、磷、钾、钙、镁和锌含量,提高了12.6%~26.3%;增加了叶绿素、叶绿素a和叶绿素b含量,分别增加了16.3%、12.1%和11.1%;对可溶性糖和可溶性蛋白则分别提升了34.8%和18.4%;同时,AMF提高了叶片各种酶活性,分别为过氧化物酶31.3%、超氧化物歧化酶17.8%、过氧化氢酶23.3%、多酚氧化酶59.1%、苯丙氨酸解氨酶65.3%;AMF提高了叶片的净光合速率(27.7%)和蒸腾速率(31.1%)。表明AMF因分类(科、属、种)不同,对叶片生理的影响也存在差异。本研究系统评价了AMF对叶片生理的影响,丰富了AMF的功能多样性理论,为筛选改善叶片生理的高效AMF提供了切实的依据。

Leaves are important plant organs,and their contents of nutrients,photosynthetic pigments,soluble sugars,and soluble proteins,as well as the activities of various enzymes,are important parameters that characterize their physiology.Photosynthesis and transpiration are important indicators of physiological activity in leaves.Mycorrhizal fungi are soil microorganisms that form symbioses with the vast majority of plants,and they can affect leaf physiology.For this study,we selected the most widely distributed arbuscular mycorrhizal fungi(AMF)and evaluated their effects on plant leaf physiology by conducting a Meta-analysis.Most previous studies have focused on investigating the physiological functions of a single plant inoculated with one AMF.To address this issue,we used a quantitative research method and a Meta-analysis to build and analyze a database of 152 relevant papers.These analyses allowed us to evaluate the impact of AMF on plant leaf physiology,and explore the effects of AMF at different classification levels.According to the results reported in the literature,AMF significantly increased the contents of nitrogen,phosphorus,potassium,calcium,magnesium,and zinc in leaves by 12.6%to 26.3%;Increased contents of chlorophyll,chlorophyll a,and chlorophyll b by 16.3%,12.1%,and 11.1%,respectively;And increased the contents of soluble sugars and soluble proteins by 34.8%and 18.4%,respectively.Various AMF also increased the activities of enzymes in leaves,including peroxidase(by 31.3%),superoxide dismutase(by 17.8%),catalase(by 23.3%),polyphenol oxidase(by 59.1%),and phenylalanine ammonia lyase(by 65.3%).As a result,AMF increased the net photosynthetic rate and transpiration rate of leaves by 27.7% and 31.1%,respectively.The results of this Meta-analysis show that different taxa(family,genus,species)of AMF have different effects on leaf physiology.These findings represent a systematic evaluation of the impacts of AMF on leaf physiology,highlight the functional diversity of AMF,and provide a practical basis for screening efficient AMF to improve leaf physiology.