红树科陆生植物竹节树的转录组分析

Transcriptome Analysis of the Terrestrial Plant Carallia brachiata from Rhizophoraceae

为了探究竹节树(Carallia brachiata)适应环境的基因表达机制,分析了竹节树8个组织(根、茎、叶、花、胚珠、果实、种子、胚)的转录组数据。结果显示繁殖器官(果实、种子、胚)和营养器官(根、茎、叶)的基因表达呈现相反趋势,即在营养器官中高表达的基因在繁殖器官中低表达,同时大部分基因在繁殖器官中呈现低表达。8个组织的特异表达基因显著富集在35个通路中。与营养器官相比,繁殖器官的特异表达基因主要富集在一些与物质合成相关的通路中,如碳水化合物、氨基酸、脂类等物质。各组织间比较所得的特异基因主要富集在53条通路中,其中糖酵解/糖异生是一条中心通路,该通路的几个关键基因在繁殖器官中的表达显著高于营养器官。结果表明,竹节树的基因表达谱与其陆生环境以及种子繁殖方式基本一致。

To explore the molecular mechanism of the Carallia brachiata adaptation to the environment,this study analyzed the transcriptome data of eight tissues(roots,stems,leaves,flowers,ovules,fruits,seeds,embryos)of the C.brachiata.The re‐sults showed that the gene expression level showed a reverse trend in reproductive(fruit/seed/embryo)and vegetative(root/stem/leaf)organs respectively,which meant that genes were highly expressed in vegetative organs but lower in reproductive ones.The specific expression genes of 8 tissues were significantly enriched in 35 pathways.Compared with the vegetative organs(roots,stems,leaves),the specific expression genes from reproductive organs were mainly enriched in some pathways related to sub‐stance synthesis,such as carbohydrates,amino acids,lipids,etc.The specific genes obtained from the comparison between dif‐ferent tissues were mainly enriched in 53 pathways.Among them,glycolysis/gluconeogenesis is a central pathway,and the ex‐pression of several key genes related to this pathway in reproductive organs are significantly higher than that in vegetative organs.The results suggested that the gene expression profile of C.brachiata was consistent with its terrestrial environment and seed prop‐agation mode.