三种马鲛鱼快速检测方法的建立及应用

The Establishment and Application of Rapid Detection Methods for Three Species of Mackerel

为了准确快速鉴定马鲛鱼样品,本研究分别以线粒体Cytb和D-loop为靶基因,筛选出康氏马鲛、蓝点马鲛与斑点马鲛的特异性PCR扩增引物,建立了3种马鲛鱼的快速检测方法,并对检测方法的特异性、重复性和灵敏性进行了验证,随后运用该方法对市售马鲛鱼及其加工制品进行检测。结果表明,马鲛鱼的快速检测方法具有良好的特异性和重复性,灵敏性检测限低于2.000 ng·μL^(-1) DNA,运用该方法能快速检测出原料及加工产品中的马鲛鱼成分,整个过程最短用时2 h。本研究建立的3种马鲛鱼PCR快速检测方法,可实现马鲛鱼物种鉴定,满足日常检测需求,对防止水产品掺假、维护消费者权益、规范市场秩序具有重要意义。

To accurately and swiftly identify samples of Scomberomorus species,this study utilized mitochondrial Cytb and D-loop as target genes to screen specific PCR primers for Scomberomorus commerson,Scomberomorus niphonius,Scomberomorus guttatus,the rapid detection methods for three types of Scomberomorus were established,and the specificity,repeatability,and sensitivity of the detection methods were validated,subsequently,this method was used to detect commercially available Scomberomorus and its processed products.The results indicate that the rapid detection method for Scomberomorus exhibits excellent specificity and repeatability,-1 the sensitivity detection limit is below 2.000 ng·µL^(-1) of DNA,using this method allows for the rapid detection of Scomberomorus components in raw materials and processed products,with the entire process taking a minimum of 2 h.The three rapid PCR detection methods for Scomberomorus established in this study enable the identification of Scomberomorus species,meeting the requirements for routine testing,this is of significant importance in preventing adulteration in aquatic products,safeguarding consumer rights and interests,and regulating market order.