豆芽中4-氯苯氧乙酸钠和6-苄基腺嘌呤残留的降解规律研究

Degradation Rules Study of 4-Chlorophenxyacetate and 6-Benzylaminopurine in Bean Sprouts

目的:研究豆芽中植物生长调节剂4-氯苯氧乙酸钠和6-苄基腺嘌呤的降解规律。方法:通过超高效液相色谱-串联质谱法,以未检出4-氯苯氧乙酸钠和6-苄基腺嘌呤的黄豆芽、绿豆芽为空白基质,采用加标回收试验研究样品在冷藏和冷冻条件下4-氯苯氧乙酸钠和6-苄基腺嘌呤的动态残留量。结果:冷冻条件下,黄豆芽、绿豆芽中4-氯苯氧乙酸钠降解半衰期分别为44.1 d、90.0 d,6-苄基腺嘌呤降解半衰期分别为32.4 d、40.8 d。冷藏条件下,黄豆芽、绿豆芽中4-氯苯氧乙酸钠降解半衰期分别为37.5 d、45.3 d,6-苄基腺嘌呤降解半衰期分别为11.8 d、31.8 d。结论:该研究为豆芽中4-氯苯氧乙酸和6-苄基腺嘌呤因自身降解造成初检结论被推翻的情况提供了理论参考。

Objective:To study the residues degradation rule of plant growth regulators 4-chlorophenxyacetate and 6-benzylaminopurine in bean sprout.Method:The ultra-high performance liquid chromatography-tandem mass spectrometry method was used to study the dynamic residues of sodium 4-chlorophenoxyacetate and 6-benzyladenine in soybean sprouts under refrigeration and freezing conditions by standard addition recovery test,and soybean sprouts and mung bean sprouts without detected 4-chlorophenoxyacetic acid sodium and 6-benzyladenine were used as blank matrices.Result:Under freezing conditions,the degradation half-lives of 4-chlorophenxyacetate in soybean sprouts and mung bean sprouts were 44.1 d and 90.0 d,respectively,and the degradation half-lives of 6-benzylaminopurine were 32.4 d and 40.8 d,respectively.Under cold storage conditions,the degradation half-lives of 4-chlorophenxyacetate in soybean sprouts and mung bean sprouts were 37.5 d and 45.3 d,respectively,and the degradation half-lives of 6-benzylaminopurine were 11.8 d and 31.8 d,respectively.Conclusion:The study provide a theoretical study on the situation that the preliminary detection conclusion is overturned due to the degradation of bean sprouts itself.