UPLC-MS/MS测定猪肉中头孢类药物的残留量

Determination of Cephalosporin Residues in Pork by UPLCMS/MS

本文采用超高效液相色谱-串联质谱对猪肉中头孢类药物残留量进行分析。样品经乙腈-水溶液和磷酸盐缓冲溶液提取,HLB固相萃取柱纯化后用超高效液相色谱质谱联用仪进行测定。结果表明,3种头孢类药物浓度在0~200 ng·mL^(-1)时,相关系数大于0.996,线性关系良好;检出限为0.001~0.365μg·kg^(-1),定量限为0.003~1.220μg·kg^(-1)。不同浓度下样品的加标回收率为83.2%~95.2%,相对标准偏差为1.9%~7.5%,标准偏差为0.078~2.817μg·kg^(-1)。

This article uses ultra high performance liquid chromatography tandem mass spectrometry to analyze the residual levels of cephalosporins in pork.The sample was extracted by acetonitrile aqueous solution and phosphate buffer solution,purified by HLB solid-phase extraction column,and determined by ultra-high performance liquid chromatography mass spectrometry.The results showed that the correlation coefficient(R2)of the three cephalosporin drugs was greater than 0.996 within 0~200 ng·mL^(-1),indicating a good linear relationship;the detection limit is 0.001~0.365μg·kg^(-1),with a quantification limit is 0.003~1.220μg·kg^(-1).The spiked recovery rates of samples at different concentrations is 83.2%~95.2%,with a relative standard deviation is 1.9%~7.5%and a standard deviation is 0.078~2.817μg·kg^(-1).