纳米硒联合聚维酮碘对手术感染杀菌的效果

Bactericidal Effect of Selenium Nanoparticles Combined with Povidone-iodine on Pathogenic Bacteria in Surgical Site Infection

【目的】负载多功能纳米硒聚维酮碘(PVP-I@Se)消毒剂对金黄色葡萄球菌(SA)、耐甲氧西林金黄色葡萄球菌(MRSA)的杀菌效果,为皮肤手术部位消毒提供实验依据。【方法】实验分为PVP-I组(对照组)与PVPI@Se组(实验组),两组的碘浓度梯度相同,分别为50、75、100、200和400μg/mL,实验组硒浓度2μg/mL,对照组不添加硒,比较两组不同的消毒液对SA、MRSA的消毒效果,检测指标包括最低抑菌浓度(MIC)和最低杀菌浓度(MBC);碘浓度50μg/mL时的最短杀菌时间;碘浓度200μg/mL、400μg/mL时的抑菌圈大小。【结果】对照组对SA、MRSA的MIC均为79.17μg/mL、实验组分别为54.17和70.83μg/mL。而对照组对SA、MRSA的MBC分别为129.17和150.00μg/mL,实验组分别为70.83和87.50μg/mL。碘浓度50μg/mL时,对照组对SA、MRSA的最短杀菌时间为130 s、140 s,实验组分别为65 s、75 s。碘浓度200μg/mL时,对照组对SA、MRSA的抑菌圈直径分别为7.67 mm、8.33 mm,实验组均为9.50 mm。碘浓度400μg/mL时,对照组对SA、MRSA的抑菌圈直径分别为9.00 mm、9.33 mm,实验组分别为11.67 mm、12.00 mm。【结论】在聚维酮碘浓度为50、75、100、200和400μg/mL时,分别加入浓度为2μg/mL的硒,可增强其对SA、MRSA的杀菌效果、缩短作用时间。硒(SeNPs)加入PVP-I对SA、MRSA的杀菌效果均有增效作用。但增敏效果SA较MRSA显著。MRSA的增效作用对碘浓度(400μg/mL)需求较高。为临床精准选用消毒剂的浓度和作用时间,降低临床皮肤手术部位感染提供理论依据。

【Objective】To investigate the bactericidal effect of loaded multifunctional povidoneiodine-nanometer sele⁃nium(PVP-I@Se)disinfectant on Staphylococcus aureus(SA)and methicillin-resistant Staphylococcus aureus(MRSA),and to provide an experimental basis for the reduction of surgical site infection(SSI).【Methods】The control group was the povidone iodine(PVP-I)group with different concentrations of iodine(50,75,100,200 and 400μg/mL).The PVPI@Se group(experimental group)was the PVP-I group further supplemented with 2μg/mL Selenium nanoparticles(SeNPs).Then we compared the bactericidal effect of the two groups of disinfectant solutions on SA and MRSA by examin⁃ing the minimum inhibitory concentration(MIC),minimum bactericidal concentration(MBC),the shortest sterilization time at a concentration of 50μg/mL iodine and the inhibition zone diameters at concentrations of 200μg/mL and 400μg/mL iodine.【Results】MIC values of PVP-I against SA and MRSA were both 79.17μg/mL,and those of PVP-I@Se were 54.17 and 70.83μg/mL,respectively.MBC values of PVP-I against SA and MRSA were 129.17 and 150.00μg/mL,re⁃spectively,and those of PVP-I@Se were 70.83 and 87.50μg/mL,respectively.At a concentration of 50μg/mL iodine,the shortest sterilization time of PVP-I for SA and MRSA was 130 s and 140 s,respectively,and that of PVP-I@Se was 65 s and 75 s,respectively.At a concentration of 200μg/ml iodine,the inhibition zone diameters of PVP-I for SA and MRSA were 7.67 mm and 8.33 mm,and those of PVP-I@Se were both 9.50 mm.At a concentration of 400μg/mL iodine,the in⁃hibition zone diameters of PVP-I for SA and MRSA were 9.00 mm and 9.33 mm,and those of PVP-I@Se were 11.67 mm and 12.00 mm,respectively.【Conclusions】PVP-I with different concentrations of 50,75,100,200 and 400μg/mL io⁃dine supplemented with 2μg/mL SeNPs have better and faster bactericidal effect on SA and MRSA.When combined with SeNPs,PVP-I can enhance the bactericidal activity against SA and MRSA,but with better sensitizing effect on SA than MRSA and higher demand of iodine concentration(400μg/mL)for sensitizing effect on MRSA.This study provides a theo⁃retical basis for selecting optimal concentration and action time of the disinfectant,thus reducing SSI.