向日葵HaFATA基因的生物信息学和表达分析

Bioinformatics and Expression Analysis of HaFATA Gene in Sunflower

FATA编码脂酰-酰基载体蛋白硫酯酶A,将质体中以脂酰-ACP形式存在的脂肪酸水解成游离的脂酰-CoA和酰基载体蛋白。为了解向日葵HaFATA基因编码蛋白的结构、功能及基因表达特性,对HaFATA编码蛋白进行生物信息学和基因表达分析。预测HaFATA为碱性且为亲水性蛋白,二级结构主要是无规则卷曲,预测亚细胞定位于叶绿体。系统进化分析表明,HaFATA与同属菊科的莴苣(Lactuca sativa L.)和水飞蓟(Silybum marianum L.)FATA进化关系近,序列相似性分别为90%、87%。HaFATA表达分析结果显示,HaFATA在向日葵种子中表达最高,其次为在管状花和叶中,在茎、舌状花和根中不表达,在发育的种子中,HaFATA除在开花后12 d表达相对较低,在其他6个发育时期表达均较高,表明其对于种子发育和油脂积累可能具有重要作用。

FATA encodes lipoacyl-acyl carrier protein thioesterase A,which hydrolyzes fatty acids in the form of lipoacyl-ACP into free lipoacyl-CoA and acyl carrier protein in plastids.In order to understand the structure and function of the protein encoded by HaFATA gene and gene expression characteristics in sunflower,the bioinformatics of the protein encoded by HafabZ and gene expression were analyzed.It was predicted that the protein encoded by HaFATA was basic and hydrophilic.The secondary structure of the protein encoded by HaFATA was mainly irregular coil,and the subcellular localization was predicted in the chloroplast.Phylogenetic analysis showed that HaFATA was closely related to Lactuca sativa L.and Silybum marianum L.,which belong to the same family of compositae,with sequence similarity of 90% and 87%,respectively.The results of expression analysis of HaFATA showed that HaFATA expression was the highest in sunflower seeds,followed by tubular flowers and leaves,and was not expressed in stems,ligulate flowers and roots.In developing seeds,HaFATA expression was relatively low at 12 d after flowering,and was high at the other six development stages,indicating that it might play an important role in seed development and oil accumulation.