连翘多糖提取工艺优化及抗氧化和抑菌活性研究

A Study on Optimization of Extraction Process and Antioxidant and Antibacteria Activity of Polysaccharides from Forsythia suspensa

以连翘果实为原料,采用超声波辅助水提醇沉法提取连翘粗多糖,以连翘粗多糖提取率为考察指标,在单因素试验的基础上,采用正交试验对连翘粗多糖提取工艺进行优化,进而采用DPPH法和ABTS法考察连翘多糖体外抗氧化活性,抑菌圈法考察连翘多糖对大肠埃希菌等食物腐败菌的抑菌活性。结果表明,连翘果实多糖的最佳提取工艺为:料液比1:20(g:mL),提取温度70℃,超声功率70 W,提取时间50 min。在此条件下,连翘果实多糖提取率为1.98%。连翘粗多糖对金黄色葡萄球菌和大肠埃希菌均有良好的生长抑制作用,且对金黄色葡萄球菌的抑制作用优于大肠埃希菌。当连翘粗多糖质量浓度为2.5 mg/mL时,大肠埃希菌的抑菌圈平均直径为10.46 mm,金黄色葡萄球菌抑菌圈平均直径为11.73 mm。连翘多糖对DPPH·和ABTS·有较好的清除能力,并呈现良好的质量浓度-效应关系。连翘多糖不但对食品腐败菌有一定的抑菌活性,还具有良好的体外抗氧化活性。可见,连翘粗多糖具有开发成食品防腐保鲜剂的潜能。

With Forsythia suspensa fruit as raw material,using ultrasonic auxiliary water alcohol extract Forsythia suspensa crude polysaccharides,the yield of polysaccharides was used as the investigation index,based on the results of the orthogonal test combined with orthogonal test of Forsythia suspensa crude polysaccharides preparation process optimization,and using DPPH method and ABTS method to investigate its in vitro antioxidant activity,bacteriostatic method to investigate the antibacterial activity of Forsythia suspensa crude polysaccharides.The results showed that the best extraction process of Forsythia suspensa crude polysaccharides follows:the solid-liquid ratio was 1:20(g:mL),the extraction temperature was 70℃,the ultrasonic power was 70 W,and the extraction time was 50 min.The Forsythia suspensa crude polysaccharides extraction rate was 1.98%.The results showed that Forsythia suspensa crude polysaccharides showed good growth inhibition on S.aureus and Escherichia coli,and better inhibition on S.aureus than E.coli.At 2.5 mg/mL,the mean diameter of E.coli and 11.73 mm of S.aureus.Forsythia suspensa polysaccharide cleared DPPH·and ABTS·and showed a good mass concentration-effect relationship.Forsythia suspensa polysaccharides not only has certain antibacterial activity against food spoilage bacteria,but also has good in vitro antioxidant activity has the potential of developing into food preservation preservatives.