摘要
Dear Editor,Genome editing with clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated nuclease(Cas)-mediated technologies have revolutionized basic plant science and crop genetic improvement(Chen et al.,2019).Stable genetic transformation of CRISPR–Cas cassette(s)is the main approach to genome editing in planta.In many sexually reproducing plants,a major concern is the dispersal of genetically modified elements through pollen(Devos et al.,2005).Maize(Zea mays L.).
基金
supported by the National Key Research and Development Program of China(2020YFE0202300)
the Henan Provincial Department of Agriculture and Rural Affairs(grant#2022010201)
the earmarked fund for CARS-Maize(CARS-02-06)
the Hainan Yazhou Bay Seed Laboratory(#B21HJ0215).