槐耳颗粒通过抑制Bmi-1与CD133表达减少三阴性乳腺癌全身转移的研究

Huaier Granules(槐耳颗粒)May Reduce Systemic Metastases in Triple-Negative Breast Cancer by Inhibiting Expressions of Bmi-1 and CD133

目的研究在不同给药方式下槐耳颗粒对三阴性乳腺癌细胞的作用效果,及槐耳颗粒是否可以影响Bmi-1进而调节肿瘤细胞CD133表达以发挥抑制肿瘤细胞转移的作用。方法选择40只3~4周龄雌性裸鼠随机分成四组,预防组、预防及治疗组、治疗组分别于建模前3周、建模前及建模后3周、建模后3周口服给予槐耳清膏溶液,对照组不给予药物治疗;建模方式采用左心室注射的方法建立三阴性乳腺癌全身转移模型,建模后六周观察期后统一处死裸鼠并取标本;通过Kaplan-Meier法分析裸鼠生存时间,免疫组化法检测裸鼠转移灶内CD133及Bmi-1的表达,组间比较采用方差分析。结果预防及治疗组裸鼠的生存时间最长(MST=42d),其次为治疗组(MST=39d),再次为预防组(MST=38 d),对照组裸鼠的生存时间最短(MST=32d);预防及治疗组的Bmi-1平均表达最低,对照组的Bmi-1平均表达最高,不同给药方式与Bmi-1的表达之间存在显著性差异(P<0.05);预防及治疗组的CD133平均表达最低,对照组的CD133平均表达最高,不同给药方式与CD133的表达之间存在显著性差异(P<0.05)。结论槐耳颗粒对三阴性乳腺癌全身转移具有预防及治疗作用;槐耳颗粒可能通过激活Bmi-1凋亡基因从而减少了三阴性乳腺癌干细胞CD133的比例,进而发挥了预防及治疗三阴性乳腺癌全身转移的作用。

Objective To study the effect of Huaier Granules(槐耳颗粒)on triple-negative breast cancer cells under differ-ent modes of administration and whether Huaier Granules can affect Bmi-1 and regulate the expression of CD133 in tumor cells to achieve the purpose of inhibiting tumor cell metastasis.Methods Forty 3-4-week-old female nude mice were randomly divided into four groups,and the prevention group,prevention and treatment group and treatment group were given Huaierqing Cream(槐耳清膏)solution three weeks before modeling,three weeks before and three weeks after modeling,and three weeks after modeling,respectively,and no drug treatment was given to the control group.The modeling method used the left ventricular injection method to establish a model of triple-negative breast cancer systemic metastasis,and the nude mice were uniformly sacrificed and the specimens were taken out after the observation period of six weeks after modeling.The survival of nude mice was analyzed by Kaplan-Meier method,and the expressions of CD133 and Bmi-1 in metastases in nude mice wre detected by immunohistochemistry,and ANOVA was used for comparison between the two groups.Results The nude mice in the prevention and treatment group had the longest survival time(MST=42 d),followed by that in the treatment group(MST=39 d)and the prevention group(MST=38d)and the control group had the shortest survival time(MST=32 d).The average expression of Bmi-1 in the prevention and treatment group was the lowest,the average expression of Bmi-1 in the control group was the highest,and there was a significant difference among different administration methods(P<0.05),the average expression of CD133 in the prevention and treatment group was the lowest,and the average expression of CD133 in the control group was the highest,and there was a significant difference among different administration methods(P<0.05).Conclusion Huaier Granules has preventive and therapeutic effects on systemic metastasis of triple-negative breast cancer.It may reduce the proportion of CD133 in triple-negative breast cancer stem cells by activating the Bmi-1 apoptosis gene,thereby preventing and treating systemic metastasis of triple-negative breast cancer.