KLF10在多发性骨髓瘤患者中的表达及与预后的关系

Expression of KLF10 in patients with multiple myeloma and its relationship with prognosis

目的探究多发性骨髓瘤患者中锌指蛋白转录因子10(KLF10)的表达及其与预后的关系。方法选取2015年1月—2017年1月我院收治的多发性骨髓瘤患者126例作为观察组,选取同期在我院健康体检的健康者120例作为对照组。采用酶联免疫吸附法(ELISA)检测受试者血清KLF10水平;采用实时荧光定量PCR(qRT-PCR)技术检测受试者血清miR-21表达水平;采用Pearson相关性分析法进行多发性骨髓瘤患者血清KLF10、miR-21水平的相关性分析;采用Kaplan-Meier法分析患者血清KLF10、miR-21水平与多发性骨髓瘤患者预后的关系;采用多因素COX回归分析影响多发性骨髓瘤患者预后的危险因素。结果观察组血清KLF10水平低于对照组,血清miR-21水平高于对照组,差异均具有统计学意义(P<0.05);Pearson相关性分析显示,观察组血清KLF10、miR-21水平呈负相关(r=-0.447,P<0.05);多发性骨髓瘤患者血清KLF10、miR-21水平与患者性别、年龄、免疫球蛋白分型无关(P>0.05),与患者DS分期、是否重度贫血、ECOG评分以及骨髓浆细胞比例有关(P<0.05);Kaplan-Meier生存曲线分析表明,多发性骨髓瘤患者血清KLF10低表达组生存率低于高表达组(χ^(2)=9.651,P=0.032);血清miR-21高表达组生存率低于低表达组(χ^(2)=12.056,P=0.0024);多因素COX回归分析显示,血清KLF10低表达、血清miR-21高表达、DS分期Ⅲ期、重度贫血、ECOG评分>3分和骨髓浆细胞比例>30%是影响多发性骨髓瘤患者发生不良预后的危险因素(P<0.05)。结论多发性骨髓瘤患者血清KLF10表达下调,其表达水平与患者临床病理特征相关,是多发性骨髓瘤患者发生不良预后的危险因素。

Objective To investigate the expression of zinc finger protein transcription factor 10(KLF10)in patients with multiple myeloma and its relationship with prognosis.Methods A total of 126 patients with multiple myeloma admitted to our hospital from January 2015 to January 2017 were gathered as the observation group.120 healthy people who underwent physical examination in our hospital during the same period were gathered as the control group for the study.The serum KLF10 level of subjects was detected by enzyme-linked immunosorbent assay(ELISA).The quantitative real-time PCR(qRT-PCR)technology was applied to detect the serum miR-21 expression level of subjects.Pearson correlation analysis method was used for correlation analysis of serum KLF10 and miR-21 levels in patients with multiple myeloma.Kaplan-Meier method was applied to analyze the relationship between serum KLF10 and miR-21 levels and prognosis of patients.The multivariate COX regression was used to analyze risk factors affecting the prognosis of patients with multiple myeloma.Results The results of this study showed that the serum KLF10 level in the observation group was lower than that in the control group,and the serum miR-21 level in the observation group was higher than that in the control group(P<0.05).Pearson correlation analysis showed that serum KLF10 and miR-21 levels in the observation group were negatively correlated(r=-0.447,P<0.05).The serum KLF10 and miR-21 levels in patients with multiple myeloma were not related to patients′gender,age,and immunoglobulin type(P>0.05),but were related to DS stage,severe anemia,ECOG score,and bone marrow plasma cell ratio of patients(P<0.05).Kaplan-Meier survival curve analysis showed that the survival rate of patients with multiple myeloma in the low-expression group of serum KLF10 was lower than that in the high-expression group(χ^(2)=9.651,P=0.032).The survival rate of the high expression group of serum miR-21 was lower than that of the low expression group(χ^(2)=12.056,P=0.0024).The multivariate COX regression analysis showed that low expression of serum KLF10,high expression of serum miR-21,DS stage III,severe anemia,ECOG score>3 points and bone marrow plasma cell ratio>30%were risk factors for poor prognosis in patients with multiple myeloma(P<0.05).Conclusion The expression of serum KLF10 in patients with multiple myeloma is down-regulated,and its expression level is related to the clinicopathological characteristics of patients,and it is a risk factor for poor prognosis in patients with multiple myeloma.