灯盏花胶囊对高脂联合STZ诱导2型糖尿病肾病小鼠的治疗作用研究

The effect of Erigeron breviscapus capsule on Type 2 diabetic nephropathyof mice induced by high-fat diet combined with streptozocin

探讨灯盏花胶囊对糖尿病肾病(DN)模型小鼠的疗效.随机抽取雄性C57BL/6J小鼠8只为正常对照,剩余62只饲喂高糖高脂饲料12周后,采用腹腔注射链脲佐菌素复制DN模型;将40只成模小鼠随机分为模型对照、阳性苯那普利(10 mg/kg)以及灯盏花胶囊低(0.75 g/kg)、中(1.5 g/kg)、高剂量组(3.0 g/kg),每组8只;各组动物连续给药6周,正常和模型组灌胃等容积纯水.检测各组小鼠空腹血糖(FBG)、24 h尿蛋白及血清中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、白蛋白(ALB)、肌酐(Cre)、尿素氮(BUN)和胰岛素(FINS)水平;HE染色观察各组小鼠肾脏组织病理学变化.结果显示模型组小鼠给药期间血糖及尿蛋白水平缓慢升高,末期肾功能受到损害,血肌酐及尿素氮明显上升,病理检查发现肾小球明显肥大,系膜区扩张,基膜增厚;各组给药6周后,小鼠FBG、24 h尿蛋白及血清中BUN、CRE、TC、TG、LDL、FINS水平和肝肾指数较模型组明显降低(p<0.05/0.01),ALB明显提升(p<0.05/0.01),肾组织病变较模型组有所减轻.本研究表明灯盏花胶囊可以明显改善高糖高脂饮食联合链脲佐菌素诱发的2型糖尿病肾病小鼠的肾脏损害,一定程度保护肾功能,延缓肾功能减退.

The aim of the study is to explore the effect of Erigeron breviscapus capsule on diabetic nephropathy mice.C57BL/6J mice were fed with high-fat diet(HFD)for successive 12 weeks and then intraperitoneally injected with streptozotocin(STZ)to establish the diabetic nephropathymodel.After that,mice were randomly divided into model group,positive control(benazepril,10 mg/kg)and three doses of Erigeron breviscapus capsule(0.75,1.5 and 3 g/kg),eight mice each group.Meantime,another eight non-HFD mice was selected as normal group,only fed with standard diet.After six weeks of continuous administration of the test substances,these parameters including fasting blood glucose(FBG),24 h urinary protein and serum triglyceride(TG),total cholesterol(TC),low density lipoprotein(LDL),albumin(ALB),creatinine(Cre),urea nitrogen(BUN)and fasting insulin(FINS)levels were detected.HE staining was used to determine the pathological changes of kidney.Results showed thatFBG,urinary protein,Cre and BUN levels increased significantly as accompanied by the injury of end-stage renal function after the induction of HFD combined with STZ.Simultaneously,glomerular hypertrophy,mesangial expansion and basement membrane thickening were observed through pathological examination.As expected,these indexes were improved on the Erigeron breviscapus capsule treated mice as well as the kidney ratios decreasing.Overall,Erigeron breviscapus capsule has the potential for treating type 2 diabetic nephropathy mainly manifested as protecting renal function to a certain extent,and delaying the decline of renal function.