子宫内膜异位症患者卵泡液外泌体miRNA谱差异及生信分析

Differential miRNA spectrum and bioinformatics analysis of follicular fluid exosomes in patients with endometriosis

目的探讨子宫内膜异位症(EMT)患者卵泡液来源的外泌体差异微小RNA(miRNA)对卵母细胞质量的影响。方法收集2021年12月—2022年3月在广州市第一人民医院生殖医学中心进行体外受精-胚胎移植/卵细胞浆内单精子注射助孕的20例不孕症患者的卵泡液,分为EMT组(EMT不孕症患者10例)和对照组(单纯男性因素不孕症患者10例)。采用高通量测序对卵泡液外泌体微小RNA(miRNA)谱进行分析,选出具有组间差异的miRNAs。结果与单纯男性因素不孕患者相比,EMT组有18个外泌体miRNAs差异有统计学意义,其中上调9个、下调9个。靶基因预测并采用GO和KEGG富集分析发现,这些靶基因主要参与磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K-Akt)、核苷酸结合寡聚结构域NOD样受体、Ras等信号通路。结论EMT患者卵泡液来源的外泌体miRNA存在差异,差异的外泌体miRNAs可能通过多个信号通路影响EMT患者卵母细胞质量。

Objective To investigate the effect of differential microRNA(miRNA)derived from follicular fluid exosomes on oocyte quality in patients with endometriosis(EMT).Methods Follicular fluid was collected from 20 infertile patients undergoing IVF-ET/ICSI in the Reproductive Medicine Center of Guangzhou First People’s Hospital from December 2021 to March 2022,including EMT group(10 patients with EMT infertility)and control group(10 patients with simple male factor infertility).The miRNA spectrum in follicular fluid exosomes was analyzed by high-throughput sequencing and miRNAs with differences between groups were selected.Results Compared with patients with infertility due to simple male factors,there were significant differences in 18 exosomal miRNAs in the EMT group,of which 9 were up-regulated and 9 were down-regulated.GO and KEGG enrichment analysis showed that these target genes were mainly involved in phosphatidylinositol-3-kinase/protein kinase B,Nucleotide binding oligomerization domain-like receptor and other signaling pathways.Conclusions There are differences in follicular fluid-derived exosomal miRNAs in EMT patients.Differential exosomal miRNAs may affect oocyte quality in EMT patients through multiple signaling pathways.