基于重测序的BBLa过表达烟草雄性不育变异检测分析

Analysis of Male Sterility Variation of BBLa Overexpressed Tobacco Based on Re-sequencing Technology

BBLa基因编码小檗碱桥状酶(Berberine bridge enzyme-like, BBLa),烟草小檗碱桥状酶参与烟碱生物合成的最终催化步骤。课题组前期研究发现,BBLa过表达烟草T0代植株中出现雄蕊败育表型。为进一步了解该雄性不育表型的遗传机制,本研究以过表达BBLa烟草雄性不育表型OE1和野生型烟草WT为材料,基于全基因组重测序技术,对单核苷酸多态性(SNP)、插入-缺失(InDel)及染色体结构变异(SV)3种变异类型进行标记注释,平均测序深度≥14×,平均覆盖率≥95%。结果表明,在2个样本中共检测到7 505 620个SNP、628 034个InDel、79 576个SV。通过2个样本的SNP差异比对筛选,最终得到174个候选基因。

BBLa gene encodes Berberine bridge enzyme-like,and this enzyme is involved in the last catalytic step of nicotine biosynthesis.In the previous study,It was found that stamen abortion phenotype appeared in T 0 generation plants of BBLa overexpressed tobacco.In order to further understand the genetic mechanism of this male sterility phenotype,based on genome-wide re-sequencing technology,this study used BBLa overexpressed tobacco male sterility phenotype OE1 and wild type(WT)tobacco as materials to mark and annotate three variation types:single nucleotide polymorphism(SNP),insertion-deletion(InDel)and chromosome structural variation(SV),with an average sequencing depth over 14×and an average coverage rate over 95%.The results showed that 7505620 SNP,628034 InDel and 79576 SV were detected in two samples.Through the comparison SNP differences between the two samples,174 candidate genes were finally obtained.