血清miR-134-5p、miR-181a-5p联合检测对良恶性肺结节的鉴别价值

The combined detection value of serum miR-134-5p and miR-181a-5p in the differential diagnosis of benign and malignant pulmonary nodules

目的 探究血清微小RNA-134-5p(miR-134-5p)、微小RNA-181a-5p(miR-181a-5p)联合检测对良恶性肺结节的鉴别价值。方法 2021年1月~2021年12月本院就诊治疗的肺结节病人124例,根据病理结果分为良性组62例、恶性组62例;采用实时荧光定量PCR(qRT-PCR)测定血清miR-134-5p、miR-181a-5p水平;采用Pearson分析血清miR-134-5p与miR-181a-5p相关性;采用多因素Logistic回归分析病人肺结节为恶性的影响因素;采用受试者工作特征曲线(ROC)评价血清miR-134-5p、miR-181a-5p在鉴别肺结节病人为良恶性中的价值。结果 恶性组肺结节病人血清miR-134-5p、miR-181a-5p水平较良性组显著降低(P<0.05);肺结节病人血清miR-134-5p水平与miR-181a-5p呈正相关(r=0.547,P<0.05);恶性肺结节病人血清miR-134-5p、miR-181a-5p水平与淋巴结是否转移、结节直径相关(P<0.05);miR-134-5p、miR-181a-5p为病人肺结节为恶性的独立保护因素,结节直径为独立危险因素(P<0.05);ROC分析显示,血清miR-134-5p、miR-181a-5p水平联合诊断的AUC为0.931,均优于两者单独鉴别(P<0.05)。结论 恶性肺结节病人血清miR-134-5p、miR-181a-5p水平较良性病人均显著降低,两者联合检测对良恶性肺结节的鉴别具有重要价值。

Objective To explore the differential value of combined detection of serum microRNA-134-5p(miR-134-5p)and microRNA-181a-5p(miR-181a-5p)in benign and malignant pulmonary nodules.Methods A total of 124 patients with pulmonary nodules who were treated in our hospital from January 2021 to December 2021 were collected as the study subjects.According to pathological results,they were grouped into a benign group of 62 cases and a malignant group of 62 cases;real-time fluorescence quantitative PCR(qRT-PCR)was applied to measure serum levels of miR-134-5p and miR-181a-5p;Pearson was applied to analyze the correlation between serum miR-134-5p and miR-181a-5p;multivariate Logistic regression was applied to analyze the influencing factors of malignant pulmonary nodules in patients;receiver operating characteristic curve(ROC)was applied to evaluate the value of serum miR-134-5p and miR-181a-5p in distinguishing between benign and malignant pulmonary nodules in patients.Results The serum levels of miR-134-5p and miR-181a-5p in patients with malignant pulmonary nodules were obviously lower than those in the benign group(P<0.05);there was a positive correlation between serum miR-134-5p level and miR-181a-5p level in patients with pulmonary nodules(r=0.547,P<0.05);the serum levels of miR-134-5p and miR-181a-5p in patients with malignant pulmonary nodules were correlated with lymph node metastasis and nodule diameter(P<0.05);miR-134-5p and miR-181a-5p were independent protective factors for malignant pulmonary nodules in patients,while nodule diameter was an independent risk factor(P<0.05);ROC analysis showed that the area under the curve(AUC)for combined diagnosis of serum miR-134-5p and miR-181a-5p levels was 0.931,both of which were superior to their individual differentiation(P<0.05).Conclusion The serum levels of miR-134-5p and miR-181a-5p in patients with malignant pulmonary nodules are obviously lower than those in benign patients.The combined detection of the two has important value in distinguishing between benign and malignant pulmonary nodules.