circ_0000502靶向miR-1231调控甲状腺乳头状癌TPC-1细胞增殖和凋亡

circ_0000502 regulates the proliferation and apoptosis of papillary thyroid cancer cell line TPC-1 by targeting miR-1231

目的探讨circ_0000502对甲状腺乳头状癌TPC-1细胞增殖、迁移、侵袭和凋亡的影响。方法选取26例甲状腺乳头状癌组织及相应癌旁组织。将TPC-1细胞分为NC组、si-NC组、si-circ_0000502组、miR-NC组、miR-1231组、si-circ_0000502+anti-miR-NC组、si-circ_0000502+anti-miR-1231组。qRT-PCR检测circ_0000502和miR-1231的表达水平;MTT检测细胞活性;Transwell、流式细胞术检测细胞的迁移、侵袭和细胞凋亡情况;Western blotting检测增殖细胞核抗原(PCNA)、基质金属蛋白酶(MMP)-2、MMP-9、B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)蛋白表达;双荧光素酶报告基因实验检测circ_0000502和miR-1231的靶向关系。结果甲状腺乳头状癌组织和细胞中circ_0000502呈高表达,miR-1231呈低表达。沉默circ_0000502或过表达miR-1231降低了TPC-1细胞增殖活力、迁移、侵袭细胞数,抑制PCNA、MMP-2、MMP-9、Bcl-2蛋白表达,促进细胞凋亡和Bax蛋白表达(P<0.05)。circ_0000502靶向调控miR-1231表达(P<0.05),下调miR-1231表达逆转了沉默circ_0000502对TPC-1细胞增殖、迁移、侵袭和凋亡的影响(P<0.05)。结论沉默circ_0000502通过靶向上调miR-1231调控TPC-1细胞的增殖、迁移、侵袭和凋亡。

Aim To investigate the effects of circ_0000502 on the proliferation,migration,invasion and apoptosis of TPC-1 cells in papillary thyroid cancer.Methods 26 cases of papillary thyroid cancer tissues and corresponding paracancer tissues were selected.TPC-1 cells were divided into NC group,si-NC group,si-circ_0000502 group,miR-NC group,miR-1231 group,si-circ_0000502+anti-miR-NC group and si-circ_0000502+anti-miR-1231 group.qRT-PCR was used to detect the expression levels of circ_0000502 and miR-1231.MTT was used to detect cell viability.Cell migration and invasion were detected by Transwell system.apoptosis was detected by flow cytometry.The expressions of proliferating cell nuclear antigen(PCNA),matrix metalloproteinase(MMP)-2,MMP-9,B cell lymphoblastoma-2(Bcl-2)and Bcl-2-associated X protein(Bax)were detected by Western blotting.The targeting relationship between circ_0000502 and miR-1231 was detected by dual-luciferase reporter gene assay.Results The expression levels of circ_0000502 was high and the expression of miR-1231 was low in papillary thyroid cancer tissues and cells.Silencing circle_0000502 or overexpression of miR-1231 reduced the proliferative vitality,migration,and invasion number of TPC-1 cells,inhibited the expression of PCNA,MMP-2,MMP-9,and Bcl-2 proteins,promoted cell apoptosis,and Bax protein expression(P<0.05).Downregulation of miR-1231 expression reversed the effects of silencing circ_0000502 on TPC-1 cell proliferation,migration,invasion and apoptosis(P<0.05).Conclusion Silencing circ_0000502 regulates the proliferation,migration,invasion and apoptosis of TPC-1 cells by targeting up-regulation of miR-1231.