宫颈癌患者癌组织c-Met mRNA与NF-κB mRNA表达的关系及其对预后的影响

Relationship between c-Met mRNA and NF-κB mRNA expression and its impact on prognosis in patients with cervical cancer

目的分析肝细胞生长因子受体原癌基因c-Met mRNA与核因子-κB(NF-κB)mRNA在宫颈癌发病中的关系及对宫颈癌患者预后的影响。方法选取本院宫颈癌患者89例作为癌变组,宫颈癌前病变患者89例作为癌前病变组,因子宫肌瘤行全子宫切除患者89例作为正常宫颈组。采用RT-PCR检测3组宫颈组织中c-Met、NF-κB mRNA相对表达量。采用Spearman/Pearson分析c-Met mRNA与NF-κB mRNA在宫颈癌发病中的关系。采用Kaplan-Meier曲线分析不同c-Met mRNA、NF-κB mRNA表达水平患者的3年生存率。结果宫颈组织中c-Met、NF-κB mRNA相对表达量,癌变组>癌前病变组>正常宫颈组(P<0.05)。癌前病变组、癌变组c-Met mRNA与NF-κB mRNA呈正相关(P<0.001)。c-Met高表达与NF-κB mRNA高表达在宫颈癌发病中呈正向交互作用(P<0.05)。c-Met、NF-κB mRNA与宫颈癌临床分期、淋巴结转移、脉管浸润呈正相关,与分化程度呈负相关(P<0.05)。癌变组c-Met、NF-κB mRNA高表达患者3年生存率低于低表达患者(P<0.05)。结论c-Met mRNA与NF-κB mRNA高表达在宫颈癌发病中呈正向交互作用,与临床分期、淋巴结转移、脉管浸润呈正相关,且宫颈癌患者3年生存率显著降低。

Aim To analyze the relationship between the mRNA of human proto-oncogene c-Met and nuclear transcription factor kappa B(NF-κB)in the pathogenesis of cervical cancer and the effect on prognosis of cervical cancer patients.Methods 89 patients with cervical cancer were selected as the cancer group.89 patients with precancerous cervical lesions were selected as the precancerous group,and 89 patients who underwent total hysterectomy due to uterine fibroids were selected as the normal cervix group.The relative expression of c-Met and NF-κB mRNA in cervical tissues of the three groups was detected by RT-PCR.Spearman/Pearson was used to analyze the relationship between these two in the development of cervical cancer.Kaplan-Meier curves were used to analyze the 3-year survival rates of patients with different c-Met and NF-κB mRNA expression levels.Results The relative expression of c-Met and NF-κB mRNA in cervical tissue was cancer group>precancerous group>the normal cervical group(P<0.05).The c-Met mRNA and NF-κB mRNA in the precancerous group and cancerous group were positively correlated(P<0.05).The high expression of c-Met mRNA and NF-κB mRNA showed a positive interaction in the pathogenesis of cervical cancer(P<0.05).The high expression of c-Met and NF-κB mRNA was positively correlated with the clinical stage,lymph node metastasis,and vascular invasion of cervical cancer,and negatively correlated with the degree of differentiation(P<0.05).The 3-year survival rate of patients with high expression of c-Met and NF-κB mRNA in the cancer group was lower than that of patients with low expression(P<0.05).Conclusion The high expression of c-Met mRNA and NF-κB mRNA has a positive interactive effect in the pathogenesis of cervical cancer,and is significantly correlated with clinical stage,lymph node metastasis,and vascular invasion.It also significantly reduces the 3-year survival rate of cervical cancer patients.