脆肉鲩器官组织胶原纤维染色观察

Observation of Collagen Fiber Staining in Organs and Tissues of Crisp Grass Carp(Ctenopharyngodon idella)

为研究饲喂蚕豆的脆肉鲩(Ctenopharyngodon idella)与普通草鱼不同器官组织中胶原纤维分布的特征,本试验对体质量7.0 kg的脆肉鲩和体质量4.5 kg的普通草鱼背部肌肉、腹部肌肉、心脏、肝胰脏、脾脏、前肠、中肠及后肠组织制作Masson染色切片,分析胶原纤维数量及分布。结果显示:器官组织中胶原纤维被染成蓝色,肌纤维被染成红色。脆肉鲩背肌、腹肌的胶原纤维染色更深、分布面积更大;背肌横切面、腹肌横切面的胶原纤维容积分数分别是普通草鱼的1.27倍和2.09倍,显示出脆肉鲩肌肉组织中胶原纤维含量更丰富;脆肉鲩肝胰脏、脾脏和心脏组织中,胶原纤维主要分布在肝胰脏门管区中的动脉、静脉管壁间隙、脾脏脾索内及心脏结缔组织中。对比发现,普通草鱼和脆肉鲩肠道组织平滑肌的大量胶原纤维分布在平滑肌细胞间,且脆肉鲩前、中、后肠的胶原纤维容积分数分别是普通草鱼的2.16倍、1.68倍和2.66倍。与肌肉、肝胰脏、脾脏和心脏相比,肠道的胶原纤维含量显著增多。器官组织的纵切和横切片显示,肌肉(骨骼肌细胞)、肠道(平滑肌细胞)和心脏(心肌细胞)的肌细胞类型并未发生改变,改变还是胶原纤维数量与分布、结缔组织的结构等。

In order to study the characteristics of collagen fiber distribution in different organs of grass carp Ctenopharyngodon idella fed with broad beans In this experiment,Masson stained sections were made on the back muscles,abdominal muscles,heart,hepatopancreas,spleen,foregut,midgut and hindgut tissues of 7.0 kg crisped grass carp and 4.5 kg common grass carp,and the quantity and distribution of collagen fibers were analyzed.The results showed that collagen fibers in the organ tissue were dyed blue and muscle fibers were dyed red.The collagen fibers of the back muscle and abdominal muscle of crisped grass carp are stained more deeply and their distribution area is larger.The volume fraction of collagen fibers in the cross section of back muscle and abdominal muscle is 1.27 times and 2.09 times of that in grass carp,respectively,indicating that the muscle tissue of crisped grass carp has more collagen fibers.In the hepatopancreas,spleen and heart tissues of crisped grass carp,the collagen fibers are mainly distributed in the arterial and venous wall space in the portal area of hepatopancreas,the splenic cord and the heart connective tissue.A large number of collagen fibers in the smooth muscle of normal grass carp and the intestinal tissue of crisped grass carp are distributed among the smooth muscle cells.The volume fraction of collagen fibers in the front,middle and rear intestines of crisped grass carp is 2.16 times,1.68 times and 2.66 times of that in grass carp,respectively.Compared with muscle,hepatopancreas,spleen and heart,the gut contains significantly more collagen fibers.The longitudinal and transverse sections of organ tissues showed that the types of muscle cells in muscle(skeletal muscle cells),intestine(smooth muscle cells) and heart(cardiomyocytes) did not change,but the number and distribution of collagen fibers and the structure of connective tissue were changed.