萝卜黑腐病的病原鉴定及室内药剂筛选

Pathogen Identification of Radish Black Rot and Fungicides Screening in Laboratory

在天津萝卜种植区发现一种严重危害萝卜的腐烂病,从发病组织中分离到5株细菌菌株,经过形态观察、致病性测定和16S rDNA序列分析研究病原种类,并采用抑菌圈法评价病原对20种常见杀菌剂的室内抑菌效果,以期为该病害的诊断和防治提供参考依据。结果表明,病原为野油菜黄单胞杆菌野油菜黑腐病致病变种(Xanthomonas campestris pv. campestris);不同杀菌剂间抑菌效果存在差异,供试的20种杀菌剂中13种杀菌剂对该病原有抑制作用,其中仅0.3%四霉素水剂和80%乙蒜素乳油对病原的抑制效果优于98%硫酸链霉素原药,27.12%碱式硫酸铜悬浮剂等7种杀菌剂对病原无抑制效果。

A serious radish rot was found in the field of Tianjin.5 bacterial isolates were isolated from diseased radish tissues.In order to provide reference for the diagnosis and prevention of this disease,pathogen identification was carried out using morphology,pathogenicity test and moleculer identification,and the toxicities of 20 kinds of bactericides were tested by inhibition zone method.The results showed that the pathogen was identified as Xanthomonas campestris pv.campestris.Antibacterial activity differed among the different agents.Among 20 kinds of the tested agents,13 agents had antibacerial effects on Xanthomonas campestris pv.campestris,of which 0.3%tetramycin aqueous solutions and 80%ethylicin emulsifiable concentrate were more effective than 98% streptomycin sulphate TC.7 agents,including 27.12%copper dihydroxosulphate suspension concentrate,had no inhibitory action.