Optimization of nucleic acid extraction methods for rapid detection in pandemic situations or diseases with high prevalence

Nucleic acid extraction(NAE)is an essential component of many assays used for diagnostic and research purposes.During a pandemic,performing NAE is associated with significant challenges due to material shortages,high costs,limited laboratory facilities,and the time required.This delays patient diagnosis and treatment,particularly in countries that do not produce NAE devices.Here,we tested several procedures to speed up the extraction process using common laboratory reagents,finding that heat-based nucleic acid extraction of severe acute respiratory syndrome coronavirus 2(SARS-COV2)is a detection method that can be easily implemented in laboratories,even those without centrifugation facilities.