甲壳素纳米纤维溶液作为内镜黏膜下注射液可行性研究

Feasibility study on chitin nanofibrils solution as an endoscopic submucosal injection

目的 探讨甲壳素纳米纤维(ChitinNFs)溶液作为内镜黏膜下注射液的可行性。方法 在离体猪胃黏膜下分别注射0.1%、0.2%的ChitinNFs溶液及生理盐水,分别记为0.1%ChitinNFs组、0.2%ChitinNFs组、对照组。应用格尺在黏膜外测量各组注射后不同时间点黏膜隆起高度。应用超声小探头收集注射后各个时间点黏膜下影像。收集注射60 min后黏膜标本进行HE染色。结果 黏膜外测量及超声影像黏膜下检测发现,0.1%ChitinNFs组、0.2%ChitinNFs组黏膜下注射后各时间点隆起高度显著高于对照组,差异有统计学意义(P<0.05)。HE染色发现,0.1%ChitinNFs组、0.2%ChitinNFs组黏膜层和固有肌层明显分离,可见注射液垫。结论 ChitinNFs溶液作为内镜黏膜下注射液具有明确的可行性。

Objective To investigate the feasibility of using chitin nanofibrils(ChitinNFs)solution as a submucosal injection.Methods 0.1%ChitinNFs solution,0.2%ChitinNFs solution and normal saline were injected into the submucosa of the isolated pig stomach,which were recorded as 0.1%ChitinNFs group,0.2%ChitinNFs group and control group,respectively.The mucosal protrusion height was measured outside the mucosa using a grid at different time points after injection.Apply a small ultrasound probe to collect submucosal images at various time points after injection.Collect mucosal samples for HE staining 60 minutes after injection.Results Extramucosal measurement and submucous examination of ultrasound image showed that the elevation height of submucous injection in 0.1%ChitinNFs group and 0.2%ChitinNFs group was significantly higher than that in control group at each time point,with statistical significance(P<0.05).HE staining showed that mucosa and muscle propria in 0.1%ChitinNFs group and 0.2%ChitinNFs group were obviously separated,and injection pad was visible.Conclusion ChitinNFs solution has clear feasibility as an endoscopic submucosal injection.