精苓鹜枣固体饮料对COPD小鼠肺部炎症因子的影响

Effects of Jingling Wuzao Solid Beverage on pulmonary inflammatory factors in mice with COPD

目的观察精苓鹜枣固体饮料对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)模型小鼠肺组织中炎症反应的影响,探讨其可能的作用机制,为该方的临床应用提供实验依据,也为中医药膳防治COPD提供一定的思路借鉴。方法将24只雄性C57BL/6小鼠随机分为正常组、模型组、精苓鹜枣组(2.5 g/kg)、地塞米松组(1.0 mg/kg),每组6只。除正常组外,其他组均采用鼻滴脂多糖联合香烟烟雾建立COPD小鼠模型。干预30 d后取材,采用HE染色观察各组小鼠肺组织及脂肪组织iWAT、BAT的病理形态学变化;ELISA检测小鼠血清和支气管肺泡灌洗液(broncho alveolar lavage fluid,BALF)中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin 6,IL-6)含量;qRT-PCR检测小鼠肺组织中TNF-α、ICAM-1、SELE、SP-A、SP-D mRNA相对表达水平。结果与正常组比较,模型组小鼠可见大量炎性细胞浸润,肺泡壁破坏融合出现肺大泡,脂肪细胞直径较大,血清及BALF中的TNF-α和IL-6的浓度显著升高(P<0.01),TNF-α、ICAM-1、SELE mRNA表达水平均显著升高(P<0.01);与模型组相比,精苓鹜枣组和地塞米松组小鼠肺组织损伤减轻,iWAT细胞变小紧致,具有棕色化趋势,BAT细胞分布增多且排列紧密,血清中TNF-α和IL-6水平均降低(P<0.05),精苓鹜枣组和地塞米松组TNF-α、ICAM-1、SELE、SP-A mRNA水平降低(P<0.05)。结论精苓鹜枣固体饮料能改善COPD模型小鼠肺组织形态,抑制炎性反应,促进肺组织修复。

Objective To observe the effects of Jingling Wuzao Solid Beverage(JLWZSB)on the inflammatory response in the lung tissue of mouse models of chronic obstructive pulmonary disease(COPD),to explore its possible mechanism of action,to provide an experimental evidence for the clinical application of this formula,and to offer some insights for the prevention and treatment of COPD with Chinese medicinal diet therapy.Methods Twenty-four male C57BL/6 mice were randomized into normal,model,JLWZSB(2.5 g/kg),and dexamethasone(1.0 mg/kg)groups,with six mice in each group.All groups except the normal group were exposed to nasal drops of lipopolysaccharide combined with cigarette smoke to establish the COPD mouse models.Samples were collected after 30 days of intervention.HE staining was used to observe the pathological morphological changes in the lung tissue and adipose tissue iWAT and BAT of each group;ELISA was employed to measure the levels of tumor necrosis factor-α(TNF-α)and interleukin 6(IL-6)in mouse serum and bronchoalveolar lavage fluid(BALF);qRT-PCR was conducted to check the relative mRNA expression levels of TNF-α,ICAM-1,SELE,SP-A,and SP-D in lung tissue of mice.Results Compared with the normal group,the model group mice showed a significant infiltration of inflammatory cells,destruction and fusion of alveolar walls resulting in pulmonary bullae,the diameter of adipocytes was larger in the model group of mice,and a significant increase in the concentrations of TNF-αand IL-6 in serum and BALF(P<0.01).The mRNA expression levels of TNF-α,ICAM-1,and SELE were significantly elevated(P<0.01).Compared with the model group,the JLWZSB and the dexamethasone groups showed reduced lung tissue damage,iWAT cells become smaller and tighter with a tendency to brown out,BAT cells become more numerous and tightly arranged in distribution,and decreased levels of TNF-αand IL-6 in serum(P<0.05),as well as reduced mRNA levels of TNF-α,ICAM-1,SELE,and SP-A(P<0.05).Conclusion JLWZSB can improve the lung tissue morphology,inhibit inflammatory responses,and promote lung tissue repair in COPD mouse models.