基于香豆素和寡聚芳酰胺的Hg^(2+)荧光探针性能及应用研究

Study on the Properties and Application of Hg^(2+)Fluorescent Probe Based on Coumarin and Oligaramide

该文利用含分子内三中心氢键的寡聚芳酰胺三聚体二酸和3-氨基香豆素的酰胺化反应,高效制备了具有一定刚性骨架的荧光探针,采用核磁共振谱、质谱等手段对其结构进行了表征。深入研究了探针的选择性识别、抗干扰以及可逆性等性能,测定了探针和Hg^(2+)的配位比及检出限,并研究了其定量检测不同水样中Hg^(2+)的能力。结果显示,探针对Hg^(2+)表现出优异的选择性,且不受其他金属离子(K^(+)、Na^(+)、Ca^(2+)、Mg^(2+)、Cu^(2+)、Zn^(2+)、Pb^(2+)、Cd^(2+)、Fe^(3+)、Mn^(2+)、Ni^(2+)、Ag+、Cr^(3+)、Al^(3+)、Co^(2+))的干扰。探针对Hg^(2+)识别具有较好的可逆性,二者配位比为1∶1,其检出限为2.08×10^(-7) mol/L,满足实际水样检测要求。利用红外光谱和核磁共振氢谱对探针识别Hg^(2+)的发光机理进行了推断。此探针可用于不同水样中Hg^(2+)的定量检测,检测结果与原子荧光法基本一致,具有较高的准确度和精密度。

A fluorescent probe with a certain rigid skeleton was prepared by amidation of oligaramide trimer diacid with intra-molecular three center hydrogen bond and 3-aminocoumarin.The structure of the probe was characterized by nuclear magnetic resonance(NMR)and mass spectrum(MS).The se⁃lective recognition,anti-interference and reversible properties of the probe were studied by fluores⁃cence emission spectra.The coordination ratio and detection limit of the probe and Hg^(2+)were deter⁃mined,and the ability of the probe to quantitatively detect Hg^(2+)in different water samples was also studied.The results showed that the probe molecule showed excellent selectivity to Hg2+in mixed so⁃lution,and was not interfered by other metal ions(K^(+)、Na^(+)、Ca^(2+)、Mg^(2+)、Cu^(2+)、Zn^(2+)、Pb^(2+)、Cd^(2+)、Fe^(3+)、Mn^(2+)、Ni^(2+)、Ag+、Cr^(3+)、Al^(3+)、Co^(2+)).The probe has good reversibility for Hg^(2+).Their coordi⁃nation ratio is 1∶1,and the detection limit is 2.08×10^(-7) mol/L,which met the requirements of actu⁃al water sample detection,and the detetion results are similar to those of atomic fluorescence meth⁃od.The luminescence mechanism of Hg2+recognition by the probe molecule was also deduced by the means of infrared spectrum(IR)and hydrogen nuclear magentic resonance(1 H NMR).The probe can be used for the quantitative detection of Hg2+in different water samples with high accuracy and preci⁃sion.