甜樱桃类钙调蛋白基因家族的生物信息学分析

Bioinformatics analysis of CML gene family in sweet cherry

类钙调蛋白(CML)是植物生长发育过程中应对刺激的一种重要蛋白,基于甜樱桃全基因组,利用生物信息学的方法对甜樱桃CML基因家族成员进行鉴定,并对理化性质、染色体定位、基因结构、启动子作用元件等进行分析。结果表明,PavCML家族有49个基因,不均匀的分布于1~8号染色体上,亚细胞定位在细胞质、叶绿体、细胞核等部位,启动子顺式作用元件分析发现PavCML家族基因响应多种元件。与转录组数据结合进行荧光定量PCR分析,结果显示CML在裂果部位的相对表达量高,表明该基因可能参与裂果过程,为CML家族基因的功能鉴定提供理论依据。

Calmodulin-like protein(CML)is an important protein in response to stimuli during plant growth and development.In this study,based on the whole genome sequence of sweet cherry,the members of sweet cherry CML gene family were identified using bioinformatics methods,and the physicochemical properties,chromosome localization,gene structure,and promoter elements were analyzed.The results showed that there were 49 genes in the PavCML family,which were unevenly distributed on chromosomes 1 to 8,and the subcellular localization was located in the cytoplasm,chloroplast and nucleus.Analysis of promoter cis-acting elements showed that PavCML family genes were involved in the response to a wide range of elements.Combined with transcriptome data,the RT-PCR results showed that the relative expression of PavCML was high in the fruit splitting site,indicating that the gene may be involved in the fruit splitting process,which provided a theoretical basis for the functional identification of CML family members.