摘要
采用多种分离手段,并结合波谱数据分析、X⁃射线单晶衍射分析和文献数据对比等方法,对滇鼠刺Itea yunnanensis的化学成分进行研究,从中分离并鉴定了7个化合物,分别为(S)⁃3⁃[1⁃(4⁃羟基苯基)丙烷⁃2⁃基]⁃4⁃甲氧基苯甲酸甲酯(1)、iteafuranal B(2)、syringaresinol(3)、二氢山柰酚(4)、均三甲氧基苯(5)、正二十烷(6)、正二十九烷(7)。其中,化合物1为新的降新木脂素类化合物,命名为iteanorneoligan A;其余化合物均为首次从该植物中分离得到。基于Sk⁃hep⁃1细胞模型,采用MTT法评价化合物的抗肝癌活性,发现化合物2对Sk⁃hep⁃1细胞表现出显著增殖抑制活性,IC_(50)为9.4μmol·L^(-1)。采用DP⁃PH、ABTS^(·+)和O^(-·)_(2)的自由基清除活性评价化合物的抗氧化潜力,发现化合物1表现出显著的ABTS·+自由基清除活性,IC_(50)为0.178 mg·mL^(-1)。
Various separation methods in combination with spectral data analysis,X⁃ray single crystal diffraction analysis,and litera⁃ture data comparison were employed to clarify the chemical constituents of Itea yunnanensis.Seven compounds were obtained from I.yunnanensis,which were identified as(S)⁃3⁃[1⁃(4⁃hydroxyphenyl)propane⁃2⁃yl]⁃4⁃methoxybenzoate methyl ester(1),iteafuranal B(2),syringaresinol(3),dihydrokaempferol(4),trimethoxybenzene(5),eicosane(6),and nonacosane(7),respectively.Among them,compound 1 was a new nor⁃neolignan compound named iteanorneoligan A,and the rest of the compounds were identified from I.yunnanensis for the first time.The anti⁃hepatocellular carcinoma effect of the compound was evaluated based on Sk⁃hep⁃1 cells model via MTT assay,and compound 2 showed a significant inhibitory effect on the proliferation of Sk⁃hep⁃1 cells with an IC_(50) of 9.4μmol·L^(-1).The antioxidant capacity was determined via DPPH,ABTS^(·+),and O^(-·)_(2)radical scavenging ability,and compound 1 ex⁃hibited a significant ABTS·+radical scavenging effect with an IC_(50) of 0.178 mg·mL^(-1).
作者
简进珍
郎天琼
张伟
封云倩
罗国勇
JIAN Jin-zhen;LANG Tian-qiong;ZHANG Wei;FENG Yun-qian;LUO Guo-yong(College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guian 550025,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2024年第6期1564-1569,共6页
China Journal of Chinese Materia Medica
基金
贵州省“千层次”创新人才项目(贵中医[ZQ2018002])
国家自然科学基金项目(81860764)
贵州省高层次人才创新创业择优项目(高层次人才择优资助合同(2021)07号)
贵阳中医学院2018年度学术新苗培养及创新探索专项(黔科合平台人才[2017]5735号⁃23)。