抗生素亚胺培南探针分子设计及传感性能研究

Design and sensing property of probe molecular for imipenem antibiotic

抗生素亚胺培南(imipenem,IPM)用药治疗败血症患者感染需要快速做出反应,目前的血药监测方法严重延迟药物注射。设计金属元素掺杂型石墨烯量子点(graphene quantum dots,GQDs),可实现荧光法快速测定IPM和开辟荧光法检测抗生素的新途径。采用水热法制备了铁掺杂GQDs(Fe-GQDs),用于快速探测IPM。采用透射电子显微镜(transmission electron microscopy,TEM)、X射线光电子能谱(X-ray photoelectron spectroscopy,XPS)以及荧光光谱等方法,探讨不同铁源起始剂对GQDs荧光性能及传感性能的影响,并分析了乙酸铁为铁源制备的Fe-GQDs的微观结构及荧光性能。结果表明:以乙酸铁为起始剂制备的Fe-GQDs荧光性能最佳,且对IPM具有显著的荧光淬灭效应。所制备的Fe-GQDs荧光淬灭强度(F/F_(0))与IPM浓度(concentration,C)呈线性关系,线性方程为F/F_(0)=0.983-4.23×10^(-3)C,线性相关系数(R 2)为0.997,线性范围为0.007~0.073 g/L。Fe-GQDs探针分子对血浆中常见有机分子和金属离子具有良好的抗干扰性,检测IPM误差为1.6%~4.6%。

Medication management of antibiotic imipenem(IPM)in the treatment of sepsis infection requires rapid response,however,the current monitoring methods of blood drugs severely delay drug injection.The aim of this work is to design metal element doped graphene quantum dots(GQDs),which can rapidly detect IPM by fluorescence method,opening up a new way for fluorescence detection of antibiotics.Iron doped GQDs(Fe-GQDs)were prepared by hydrothermal method for rapid detection of IPM.The effects of different precursors of iron sources on the fluorescence and sensing performance of GQDs were investigated using transmission electron microscopy(TEM),X-ray photoelectron spectroscopy(XPS),fluorescence spectroscopy,etc.The microstructure and fluorescence properties of Fe-GQDs prepared with iron acetate were analyzed.The results showed that Fe-GQDs prepared with iron acetate as the precursor exhibited the best fluorescence performance and a significant fluorescence quenching effect on IPM.The fluorescence quenching intensity(F/F_(0))of the as-prepared Fe-GQDs was linearly related to the concentration(C)of IPM,where the linear equation was F/F_(0)=0.983-4.23×10^(-3)C,with a linear correlation coefficient(R^(2))of 0.997 and a linear range of 0.007-0.073 g/L.The Fe-GQDs probe molecules delivered a good anti-interference performance against common organic molecules and metal ions in plasma with a detection error of 1.6%-4.6%for sensing IPM.