移植肾缺血/再灌注损伤后PBRM1分子表达及作用分析

Expression and effect of PBRM1 in renal transplant ischemia reperfusion injury

目的探讨PBRM1分子在移植肾缺血/再灌注损伤(ischemia reperfusion injury,IRI)中的表达及其作用机制。方法选取3组肾脏组织(每组5例),分为正常肾脏组(Control组)、移植术后肾功能稳定组(STA组)、移植肾缺血/再灌注损伤组(IRI组)。采用免疫组化技术检测多溴蛋白1(polybromo 1,PBRM1)在3组中的蛋白表达。结合基因表达数据库(Gene Expression Omnibus,GEO)数据集分析PBRM1在肾脏IRI后的表达,探讨其机制,并通过体外实验验证Pbrm1分子在Th17细胞的表达。结果免疫组化染色结果显示,与Control组和STA组相比,IRI组的PBRM1表达程度显著高于其他两组。经GSE180420数据库分析显示,与Control相比,IRI组PBRM1分子表达水平显著升高,GSEA结果提示PBRM1可促进Th17细胞的功能。体外实验证实,与Th0细胞相比,Pbrm1基因的转录水平在Th17细胞中显著升高。结论移植肾IRI后PBRM1分子表达水平显著升高,PBRM1分子可能通过影响Th17细胞的功能,从而加重肾脏IRI。

Objective To investigate the expression and scientific significance of PBRM1 in renal transplantation ischemia reperfusion injury(IRI).Methods Three groups of kidney tissues(5 cases in each group)were selected and divided into normal kidney group(Control),stable allograft function(STA group),and ischemia injury(IRI group).The expression of polybromo 1(PBRM1)in the three groups was detected by immunohistochemistry.The expression of PBRM1 after renal IRI was analyzed with Gene Expression Omnibus(GEO)data set and its mechanism was discussed.Finally,the expression of Pbrm1 in Th17 cells was verified in vitro.Results Immunohistochemical staining showed that the expression of PBRM1 in IRI group was significantly higher than that in the other two groups.GSE180420 database analysis showed that PBRM1 expression was significantly increased in IRI group compared with Control,and GSEA results suggested that PBRM1 could promote the function of Th17 cells.In vitro experiments confirmed that the transcription level of Pbrm1 was significantly increased in Th17 cells compared with Th0 cells.Conclusion The expression of PBRM1 was significantly increased after renal IRI transplantation.PBRM1 may aggravate renal IRI by affecting the function of Th17 cells.