基于16S rRNA研究铜污染对土壤细菌群落、抗生素抗性基因及基因转移的影响

A 16S rRNA-based study of the effect of copper contamination on antibiotic resistance genes and horizontal gene transfer in soil bacterial communities

对经0、200、400 mg·kg^(-1)Cu^(2+)处理的3个种植池土壤分别采样,并对所有样品进行16S rRNA高通量测序。结果表明:铜污染改变了土壤中的细菌群落结构,抗生素抗性基因(antibiotic resistance genes, ARGs)与基因水平转移(horizontal gene transfer, HGT)相关基因的丰度随Cu^(2+)浓度的升高而增加。富集的抗性基因大多为多药耐药基因。这表明铜胁迫会改变土壤中细菌群落结构,促进HGT,同时使ARGs富集。

In this study, soil samples were collected from three planting ponds treated with 0, 200 and 400 mg Cu^(2+), respectively, and all samples were sequenced by 16S rRNA high-throughput sequencing. Sequencing results showed that Cu contamination changed the bacterial community structure in soil, and the abundance of antibiotic resistance genes(ARGs) and horizontal gene transfer(HGT) related genes increased with the increase of Cu^(2+) concentration. The resulting antibiotic resistance genes were mostly multidrug resistance genes. This study showed that under the stress of Cu contamination, the bacterial community structure in soil was changed, and the HGT between bacteria was promoted, while the ARGs were enriched.