不同质量浓度碳氮源配比对谷氨酸发酵的影响

The effect of different quality concentration carbon nitrogen sources was compared with glutamic acid fermentation

通过设计不同质量浓度碳氮源配比进行谷氨酸发酵,采用单因素试验和正交试验优化谷氨酸发酵的碳氮源配比。试验结果表明,葡萄糖质量浓度0.07 g/mL,尿素质量浓度0.009 g/mL,硫酸铵质量浓度0.014 g/mL为最优碳氮源组合,其中有机氮源尿素是影响谷氨酸产量的最关键因素。碳源葡萄糖和无机氮源硫酸铵的质量浓度过高时会对谷氨酸产量产生抑制作用。

By designing different mass concentration ratios of carbon and nitrogen sources for glutamate fermentation,Single-factor experiments and orthogonal tests were used to optimize the carbon to nitrogen source ratio for glutamate fermentation.The results showed that glucose concentration of 0.07 g/mL,urea concentration of 0.009 g/mL and ammonium sulfate concentration of 0.014 g/mL were the optimal carbon and nitrogen source combinations,among which organic nitrogen source urea was the most critical factor affecting glutamic acid yield.Excessively high concentrations of the carbon source glucose and the inorganic nitrogen source ammonium sulfate can inhibit glutamate production.