金鲳鱼肠炎病的病原菌分离鉴定及其中草药防治

Isolation,Identification and Chinese Herbal Medicine Control of Enteritis Pathogenic Bacteria from Trachinotus Ovatus

从具有肠道糜烂、肛门突出体外病症的金鲳鱼(Trachinotus ovatus)体内筛选致病菌株,分离纯化获得菌株05A。通过活菌注射金鲳鱼腹腔回归感染试验,确定菌株05A为致病菌株。经单菌落形态特征、生理生化指标以及16S rRNA分子鉴定,确定其为哈维氏弧菌(Vibro harveyi)。中草药的体外抑菌实验表明,大蒜、儿茶、五倍子和石榴皮等4种中草药对病原菌05A有较好的抑菌效果,其次是仙鹤草、黄连、黄芩、甘草、大黄、五味子和诃子。

Strain 05A was isolated from Trachinotus ovatus specimens with intestinal erosion and anal outbreak.Challenge experiments using strain 05A on healthy T.ovatus and bacterial re-isolation from challenged diseased golden pompano showed that the bacteria strain named 05A induced identical pathological symptoms,indicating that it is the causative pathogen of the disease.According to the morphological characteristics,physiological and biochemical properties,and 16S rRNA sequences,the isolate 05A was identified as Vibro harveyi.Bacteriostatic test of Chinese herbal medicine revealed that garlic,catechu,gallnut and pomegranate peel had good bacteriostatic effect on the strain 05A,followed by Agrimonia pilosa,Coptis chinensis,Scutellaria baicalensis,Glycyrrhiza uralensis,Rheum palmatum,Schisandra chinensis,and Terminalia chebula.