小麦miR166基因家族鉴定及表达分析

Genome-wide identification and expression analysis of miR166 gene family in wheat

miR166作为重要的转录后调节因子,在植物的生长发育和对逆境胁迫的反应中扮演着重要的角色。利用PmiREN、Ensembl Plants数据库、MEGA-X、DNAMAN软件以及RNAfold web server、WebLogo和psRNA Target在线网站对小麦miR166(Tae-miR166)基因家族的进化特性和表达模式进行分析。在PmiREN数据库中搜索到19个Tae-miR166基因家族成员,染色体定位发现Tae-miR166成员定位于14条染色体上。序列比对发现Tae-miR166的成熟体序列高度保守。进化分析结果表明,Tae-miR166基因家族成员分别处于4个进化分支。靶基因预测结果表明,Tae-miR166的靶基因包括Ⅲ类同源异型结构域亮氨酸拉链蛋白、β半乳糖苷酶和钙依赖性蛋白激酶等。转录组数据分析表明Tae-miR166家族19个成员在小麦6个组织中都有表达,在籽粒和穗中表达量最高。荧光定量PCR结果表明,Tae-miR166家族15个成员在镉、干旱和低温胁迫处理后的表达模式存在差异,说明Tae-miR166基因家族在植株抵御非生物胁迫时发挥着重要作用。本研究为进一步阐明Tae-miR166基因家族的功能提供了理论依据。

As an essential post-transcriptional regulator,miR166 played an important role in plant growth and development and response to stress.The evolutionary characteristics and expression patterns of miR166 gene family in wheat(Tae-miR166)were analyzed by PmiREN,Ensembl Plants database,MEGA-X,DNAMAN software,RNAfold web server,WebLogo and psRNATarget online websites.Nineteen members of the Tae-miR166 gene family were searched in the PmiREN database,and chromosome localization revealed that Tae-miR166 were localized on 14 chromosomes.Sequence alignment revealed that the mature sequences of Tae-miR166 were highly conserved;evolutionary analysis showed that the Tae-miR166 gene family members were in 4 evolutionary branches,respectively.The target gene prediction results showed that the target genes of Tae-miR166 gene family included class III homologous heterotypic structural domain leucine zipper protein,β-galactosidase and calcium-dependent protein kinase.RNA-seq data analysis showed that 19 members of the Tae-miR166 family were expressed in 6 tissues of wheat,with the highest expression in grains and ears.The results of RT-qPCR showed that the expression patterns of Tae-miR166 family members were different after cadmium,drought and low temperature stress treatment,indicating that Tae-miR166 gene family played an important role in plant resistance to abiotic stress.This study provided a theoretical basis for further elucidating the function of miR166 gene family in wheat.