摘要
Biobutanol is an advanced biofuel that can be produced from excess lignocellulose via acetone-butanol-ethanol(ABE)fermentation.Although significant technological progress has been made in this field,attempts at largescale lignocellulosic ABE production remain scarce.In this study,1m^(3)scale ABE fermentation was investigated using high inhibitor tolerance Clostridium acetobutylicum ABE-P1201 and steam-exploded corn stover hydrolysate(SECSH).Before expanding the fermentation scale,the detoxification process for SECSH was simplified by process engineering.Results revealed that appropriate pH management during the fed-batch cultivation could largely decrease the inhibition of the toxic components in undetoxified SECSH to the solventogenesis phase of the ABE-P1201 strains,avoiding“acid crash”.Therefore,after naturalizing the pH by Ca(OH)_(2),the undetoxified SECSH,without removal of the solid components,reached 17.68±1.30 g/L of ABE production with 0.34±0.01 g/g of yield in 1 L scale bioreactor.Based on this strategy,the fermentation scale gradually expanded from laboratory-scale apparatus to pilot-scale bioreactors.Finally,17.05±1.20 g/L of ABE titer and 0.32±0.01 g/g of ABE yield were realized in 1m3 bioreactor,corresponding to approximately 145 kg of ABE production from 1 t of dry corn stover.The pilot-scale ABE fermentation demonstrated excellent stability during repeated operations.This study provided a simplified ABE fermentation strategy and verified the feasibility of the pilot process,providing tremendous significance and a solid foundation for the future industrialization of second-generation ABE plants.
基金
funded by the National Key Research and Development Program of China(grant number:2022YFC2106300).
