中华按蚊CCE家族基因的表达模式分析

Analysis of expression patterns of CCE family genes in Anopheles sinensis(Diptera:Culicidae)

【目的】羧酸酯酶(carboxylesterases,CCEs)是一类重要的水解酶超家族,参与昆虫体内各种外源物质的代谢过程。本研究利用不同发育阶段、不同组织和吸血前后中华按蚊Anopheles sinensis的转录组数据分析CCE基因表达模式,为进一步研究CCEs在不同生理过程中的潜在功能奠定基础。【方法】以前期从中华按蚊实验室品系转录组数据库中筛选出的50个CCE基因,利用生物信息学分析其在中华按蚊不同发育阶段(卵、1-4龄幼虫、雄蛹、雌蛹、雄成蚊和雌成蚊)、成蚊不同组织(触角、唾液腺、中肠、马氏管、精巢、卵巢、表皮和脂肪体)中以及雌成蚊吸血前和吸血后不同时间(1,3,6,12,24和48 h)的表达模式。【结果】CCE基因主要在幼虫或成蚊阶段高表达或特异性表达;AsAe12,AsAe4和AsBe4在所有发育阶段均高表达。CCE基因的表达具有组织特异性,主要集中在成蚊触角、表皮和精巢中表达;此外,5个CCE基因(AsAe13,AsAe12,AsAe6,AsAe4和AsBe4)在成蚊中肠、马氏管和脂肪体中均高表达,可能与这些解毒器官代谢异生物有关。在雌成蚊吸血后,大多数CCE基因的表达量发生了变化,它们的表达模式也存在差异,这表明血液消化是一个复杂的过程。【结论】本研究结果丰富了中华按蚊CCE基因的知识,为深入探究CCE家族基因在中华按蚊生长发育中的的潜在功能提供有价值的参考。

【Aim】Carboxylesterases(CCEs)are an important hydrolase superfamily and have a role in the metabolism of various exogenous substances in insects.This study aims to lay a foundation for further research on the potential functions of CCEs in different physiological processes by analyzing the expression patterns of CCE genes from transcriptome datasets of Anopheles sinensis at different developmental stages,in different tissues,and before and after blood meal.【Methods】Based on 50 CCE genes collected from the obtained transcriptome data of the An.sinensis laboratory strain,the expression patterns of CCE genes in An.sinensis at different developmental stages(egg,1st-4th instar larvae,male pupa,female pupa,male adult and female adult),in different adult tissues(antennae,salivary gland,midgut,Malpighian tubules,testis,ovary,cuticle and fat body),and female adults before and after blood meal(at 1,3,6,12,24 and 48 h)were analyzed by bioinformatics.【Results】CCE genes were mainly highly or specifically expressed in the larval or adult stages.AsAe12,AsAe 4 and AsBe 4 were highly expressed during all developmental stages.The expression patterns of CCE genes were also tissue specific and mainly expressed in antennae,cuticle and testis of adults.Moreover,five CCE genes(AsAe13,AsAe12,AsAe6,AsAe 4 and AsBe 4)were highly expressed in the midgut,Malpighian tubules and fat body of adults,suggesting their potential involvement in xenobiotic metabolism within these detoxification organs.After feeding female adults with blood meal,the expression levels of most CCE genes changed,and their expression patterns were different,suggesting that the blood digestion is a complex process.【Conclusion】The results of this study have enriched the knowledge of CCE genes in An.sinensis,providing a valuable reference for further research on the potential function of CCE family genes in the growth and development of An.sinensis.