小白杏生理落果中多酚提取及体外抗氧化活性分析

Study on extraction and antioxidant activity of polyphenols from Armeniaca vulgaris Lam.during physiological fruit-falling period

【目的】优化小白杏生理落果中多酚提取工艺,并研究其提取物抗氧化活性。为小白杏生理落果抗自由基氧化功能食品的开发与利用奠定基础。【方法】以生理落果小白杏为原料,在单因素试验的基础上,采用响应面试验确定最佳工艺,并用分光光度法测定多酚提取物的体外抗氧化活性。【结果】小白杏多酚的最佳提取工艺为提取时间34 min、提取温度60℃、液料比21∶1、乙醇浓度70%,小白杏生理落果多酚得率为12.50 mg/g。验证试验结果与预测值相对标准偏差为1.31%,回归模型适用于小白杏多酚的提取。小白杏多酚清除ABTS^(+)、DPPH、O_(2)^(-)的IC_(50)值分别为66.35、55.84和103.00μg/mL,其清除自由基的能力由强到弱依次为DPPH、ABTS^(+)、O_(2)^(-);小白杏生理落果多酚对NO^(2-)的清除能力较弱。【结论】小白杏生理落果多酚能不同程度地清除ABTS^(+)、DPPH、O_(2)^(-)、NO^(2-),具有一定的体外抗氧化活性,可用作抗自由基氧化功能食品的原料。

【Objective】In this study,the extraction process of polyphenols from Armeniaca vulgaris Lam.during physiological fruit-falling period was optimized and the antioxidant activity of the polyphenol extract was analyzed.【Methods】On the basis of single factor experiments,response surface method was used to determine the optimum process.The antioxidant activity in vitro was assayed with spectrophotometric method.【Results】the suitable extraction conditions were ethanol volume fraction 70%,solid-liquid ratio 1∶21,extraction time 34 minute and temperature 60℃.Under these conditions,the polyphenol extraction yield was 12.50 mg/g.The relative standard deviation of the proved experiment was 1.31%compared with the predicted value,which indicated the regression model was suitable for the polyphenol extraction from A.vulgaris.during physiological fruit-falling period.The IC_(50) of scavenging the free radicals of ABTS^(+),DPPH,O_(2)^(-)were 63.55,55.84,103.00μg/mL respectively.The results indicated that the ability of the polyphenol extraction to scavenge free radicals in the order was DPPH>ABTS^(+)>O_(2)^(-).There is slightly lower scavenging ability of NO^(2-).【Conclusion】The findings would lays the foundation for the development and utilization of A.vulgaris.during physiological fruit-falling period in anti-free radical oxidation functional food.