花生种质遗传多样性分析及指纹图谱构建

Analysis of Genetic Diversity of Peanut Germplasm and Construction of Fingerprint

本研究利用SSR标记对50份花生种质资源进行遗传多样性分析及DNA指纹图谱构建。38对SSR引物在50份种质资源中共扩增出141个基因位点,每个引物平均有3.71个位点,多态性扩增位点126个,多态性比率为89.36%,平均多态性信息含量(PIC)为0.52,变异范围为0.40~0.88;采用UPGMA法进行聚类,在GS 0.648处可将50份花生种质资源聚为4个类群,50份花生种质资源的遗传相似系数(GS)介于0.436~0.955之间,平均值为0.735。此外,利用38对SSR引物构建了一份独特的DNA指纹图谱QR编码,以期为花生新品种分类鉴定、亲缘关系分析以及种质资源的利用奠定理论基础。

In this study,SSR markers were used to analyze genetic diversity and construct DNA fingerprinting of50 peanut germplasm resources.A total of 141 gene loci were amplified by 38 pairs of SSR primers in 50 germplasm resources,with an average of 3.71 loci per primer,of which 126 were polymorphic amplified loci,with a polymorphism ratio of 89.36%,and many primers the average value of PIC is 0.52,and the variation range is0.40~0.88.Using the UPGMA method for clustering,the 50 peanut germplasm resources could be clustered into four groups at GS 0.648,and the genetic similarity coefficients(GS) of the 50 peanut germplasm resources ranged from 0.436 to 0.955,with the average value of 0.735.In addition,38 pairs of SSR primers were used to construct a unique DNA fingerprinting QR code,which will provide an important basis for the selection and identification of new peanut varieties in the future.