三种植物生长调节剂对月季离体再生的影响

Effect of Three Plant Growth Regulators on in Vitro Regeneration of Rose spp.

为了提高月季遗传转化的体细胞胚胎的植株再生率,以月季B103 (Rosa spp.)无菌苗为材料,探究不同浓度的细胞分裂素6-BA、KT、TDZ对植株生长的影响,并以优化的培养基EB和ET对成熟的月季B103体胚进行再生诱导。结果表明:添加KT的培养基(RK)上的无菌苗全部死亡,添加6-BA (RB)和TDZ(RT)的两组生长正常,最佳培养基为MS+NAA 0.05 mg/L+GA33.0 mg/L+6-BA/TDZ 0.5 mg/L+3%蔗糖+0.7%琼脂pH=5.7。成熟月季体胚在培养基MS+NAA 0.05 mg/L+GA33.0 mg/L+TDZ 0.5 mg/L+3%蔗糖+0.7%琼脂p H=5.7上诱导分化,30 d内再生出数量最多的正常植株,再生率为20%。ET组分化出丛生状态的子叶,再生率高于20%。本研究表明ET2可诱导月季细胞分化出正常再生植株。

In order to improve the plant regeneration rate of rose somatic embryos genetically transformed,the rose material B103(Rosa spp.) sterile seedling was used as the material,explore the effects of different concentrations of cytokinin 6-BA,KT,and TDZ on plant growth,and use the optimized medium RB and RT to induce regeneration of mature rose material B103 somatic embryos.The results showed that the sterile seedlings on the medium(RK) supplemented with KT all died,and the two groups supplemented with 6-BA(RB) and TDZ(RT)grew normally.The best medium is MS+NAA 0.05 mg/L+GA33.0 mg/L+6-BA/TDZ 0.5 mg/L+3% sucrose+0.7%agar pH=5.7.Mature rose somatic embryos were induced to differentiate on the medium MS +NAA 0.05 mg/L+GA33.0 mg/L+TDZ 0.5 mg/L+3% sucrose+0.7% agar pH=5.7,the largest number of normal plants regenerated within 30 days,with a regeneration rate of 20%.Cotyledons in a clumped state were formed by RT components,and the regeneration rate was higher than 20%.This study showed that ET2 could induce the differentiation of rose cells into normal regenerated plants.