干旱胁迫及钙胁迫对血人参幼苗生长的影响

Effects of Calcium Stress and Drought Stress on theGrowth of Indigofera stachyodes Seedlings

为了探讨CaCl_(2)胁迫和干旱胁迫对血人参幼苗生长的影响,分别采用不同浓度梯度的CaCl_(2)溶液和不同干旱环境对血人参幼苗进行胁迫实验。结果表明,随着土壤含水量的升高,血人参幼苗总的生物量呈上升趋势,血人参总叶绿素含量最适合的相对田间持水量为60%~65%;在极度干旱条件下,丙二醛(MDA)含量和游离脯氨酸(Pro)含量最低,过氧化氢酶(CAT)活性最强。在适宜的钙浓度(5~35 mmol/L)环境下,可以促进血人参幼苗的胚根、主根、鲜重等生长发育;在钙浓度过量时,反而会产生抑制作用。在适宜的钙胁迫浓度(5~35 mmol/L)范围内,血人参幼苗中MDA含量与叶绿素的含量有所增加,同时SOD活性、POD活性、Pro含量及CAT的活性升高。综合分析表明,钙离子浓度过高或过低均会抑制血人参幼苗的生长,其幼苗虽具有一定的抗旱性,但随着干旱程度的加深,幼苗植株的生长会受到抑制。因此,在血人参的引种繁育过程中,应注意适当的保水、补水和控制钙离子浓度。

In order to explore the effects of CaCl_(2)stress and drought stress on the growth of Indigofera stachyodes seedlings,different concentrations of CaCl_(2)solution and different drought environments were used to conduct stress experiments on Indigofera stachyodes seedlings.The results showed that under drought stress conditions,the total biomass of Indigofera stachyodes seedlings increased with the increase of soil water content.The optimal relative field water content for the total chlorophyll content of Indigofera stachyodes was 60%-65%.Under extreme drought conditions,the content of malondialdehyde(MDA)and free proline(Pro)was the lowest,and catalase(CAT)activity was the strongest.In the environment of appropriate calcium concentration(5-35 mmol/L),the growth and development of embryonic roots,main roots,fresh weight and other growth indicators of Indigofera stachyodes seedlings could be promoted.When the calcium concentration was excessive,it will had an inhibitory effect instead.In the range of appropriate calcium stress concentration(5-35 mmol/L),the content of MDA and chlorophyll increased,and the activity of superoxide dismutase(SOD),peroxidase(POD),free proline(Pro)and CAT increased.The comprehensive analysis showed that both too high and too low calcium ion concentrations will have an inhibitory effect on the growth of Indigofera stachyodes seedlings.Although the seedlings had certain drought resistance,as the degree of drought deepens,the growth of seedlings will be inhibited.Therefore,in the process of introducing and breeding Indigofera stachyodes,attention should be paid to appropriate water conservation,supplementation and control of calcium ion concentration.